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Fixed-gap tunnel junction for reading DNA nucleotides.

Pei Pang1, Brian Alan Ashcroft, Weisi Song

  • 1Biodesign Institute, ‡Department of Physics, §Department of Chemistry and Biochemistry, Arizona State University , Tempe, Arizona 85287, United States.

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|November 8, 2014
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Summary

Researchers developed a new fixed-junction device for electronic DNA nucleotide detection. This method uses a layered tunnel junction to identify individual nucleotides by analyzing fluctuating tunnel current signals during single-molecule binding events.

Keywords:
DNA sequencingchemical recognitionrecognition tunnelingtunnel junction

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Area of Science:

  • Nanotechnology
  • Molecular Biology
  • Biophysics

Background:

  • Previous electronic conductance measurements of biomolecules relied on mechanically adjusted tunnel junctions.
  • Fixed-junction devices previously lacked the resolution for chemical information at the single-molecule level.

Purpose of the Study:

  • To develop a fixed-junction device capable of identifying individual DNA nucleotides based on their electronic properties.
  • To overcome the limitations of previous methods in obtaining chemical information from single-molecule electronic measurements.

Main Methods:

  • Fabrication of a layered tunnel junction using atomic layer deposition for gap definition.
  • Utilizing reactive ion etching to create a molecular pathway through the junction.
  • Functionalization of metal electrodes with recognition molecules for specific nucleotide capture.
  • Analysis of fluctuating tunnel current signals during single-molecule binding events.

Main Results:

  • The layered tunnel junction successfully detected and identified individual DNA nucleotides in solution.
  • Characteristic fluctuations in tunnel current correlated with the binding of specific nucleotides.
  • Demonstrated a novel approach for single-molecule chemical identification using electronic transport.

Conclusions:

  • This fixed-junction device offers a promising new method for label-free, single-molecule DNA nucleotide identification.
  • The technique advances the field of molecular electronics for potential applications in diagnostics and sequencing.