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MicroRNA-21 regulates the sensitivity to cisplatin in a human osteosarcoma cell line

W Ziyan1, L Yang2

  • 1Department of Orthopedics, Fifth Hospital of Wuhan, Hubei, 430050, People's Republic of China. sanming712@126.com.

Irish Journal of Medical Science
|November 10, 2014
PubMed
Abstract

Insights

MicroRNA-21 (miR-21) overexpression in osteosarcoma increases resistance to CDDP chemotherapy. Suppressing miR-21 enhances CDDP

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • MicroRNA-21 (miR-21) is frequently overexpressed in various solid tumors.
  • miR-21 plays a role in modulating cellular resistance to chemotherapy-induced apoptosis.

Purpose of the Study:

  • To investigate the role of miR-21 in osteosarcoma cell sensitivity to cisplatin (CDDP).
  • To explore the underlying molecular mechanisms of miR-21-mediated chemoresistance.

Main Methods:

  • Osteosarcoma cells were transfected with miR-21 mimics, anti-miR-21, or bcl-2 siRNA.
  • Cellular sensitivity to CDDP was assessed following transfection and treatment.
  • The expression of bcl-2 was evaluated in conjunction with miR-21 modulation.

Main Results:

  • Overexpression of miR-21 in osteosarcoma cells conferred significant resistance to CDDP.
  • Suppression of miR-21 expression enhanced the cytotoxic effects of CDDP.
  • Down-regulation of bcl-2 using siRNA counteracted the chemoresistance induced by miR-21.

Conclusions:

  • miR-21 acts as a key regulator of CDDP chemoresistance in osteosarcoma.
  • The observed chemoresistance is mediated through the regulation of bcl-2 expression.
  • This study elucidates a novel mechanism contributing to drug resistance in osteosarcoma.
Keywords:
CisplatinDrug resistanceMicroRNA-21Osteosarcoma

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