Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Telomeres and Telomerase02:41

Telomeres and Telomerase

28.9K
In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded...
28.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Prevalence of germinal center B-cell-like and non-germinal center B-cell-like types of diffuse large B-cell lymphoma in Shanghai, China].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2010
Same author

Enhancement of exo-polysaccharide production and antioxidant activity in submerged cultures of Inonotus obliquus by lignocellulose decomposition.

Journal of industrial microbiology & biotechnology·2010
Same author

Two new dihydrobenzofuran lignans from Rabdosia lophanthoides (Buch.-Ham.ex D.Don) Hara.

Journal of Asian natural products research·2010
Same author

Biogenic silica in intertidal marsh plants and associated sediments of the Yangtze Estuary.

Journal of environmental sciences (China)·2010
Same author

Analysis of oral microbiota in children with dental caries by PCR-DGGE and barcoded pyrosequencing.

Microbial ecology·2010
Same author

Determination of bicyclol in dog plasma using liquid chromatography-tandem mass spectrometry.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2010
Same journal

Correction: A method for supervoxel-wise association studies of age and other non-imaging variables from coronary computed tomography angiograms.

Scientific reports·2026
Same journal

Poly(bromophenol blue)/CoSn(OH)<sub>6</sub> cubic particles modified pencil graphite electrode for electrochemical determination of diphenhydramine.

Scientific reports·2026
Same journal

Dietary Chlorella, Spirulina, and acidifier modulate jejunal cytokine-related gene expression in broiler chickens.

Scientific reports·2026
Same journal

Perceived physical activity barriers in university students: associations with fatigue and eating behaviours.

Scientific reports·2026
Same journal

Refuge limitation structures habitat use in agricultural landscapes: evidence from Sunda pangolins.

Scientific reports·2026
Same journal

Lightweight stateless transaction verification with outsourced witness updates for UTXO blockchains.

Scientific reports·2026
See all related articles

Related Experiment Video

Updated: Apr 21, 2026

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
12:08

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Published on: May 22, 2013

47.6K

Assessing telomere length using surface enhanced Raman scattering.

Shenfei Zong1, Zhuyuan Wang1, Hui Chen1

  • 1Advanced Photonics Center, Southeast University, Nanjing 210096, China.

Scientific Reports
|November 11, 2014
PubMed
Summary
This summary is machine-generated.

A new optical method using surface-enhanced Raman scattering (SERS) accurately measures telomere length. This SERS-based protocol simplifies detection without polymerase chain reaction (PCR) or electrophoresis, offering a cost-effective alternative for disease assessment.

More Related Videos

Author Spotlight: Optimization of Performance Parameters of the TAGGG Telomere Length Assay
08:23

Author Spotlight: Optimization of Performance Parameters of the TAGGG Telomere Length Assay

Published on: April 21, 2023

3.8K
Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
11:29

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Published on: July 10, 2017

13.7K

Related Experiment Videos

Last Updated: Apr 21, 2026

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
12:08

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Published on: May 22, 2013

47.6K
Author Spotlight: Optimization of Performance Parameters of the TAGGG Telomere Length Assay
08:23

Author Spotlight: Optimization of Performance Parameters of the TAGGG Telomere Length Assay

Published on: April 21, 2023

3.8K
Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
11:29

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Published on: July 10, 2017

13.7K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Telomere length is a critical biomarker for cellular aging and diseases like cancer.
  • Current telomere length measurement methods can be complex and time-consuming.

Purpose of the Study:

  • To introduce a novel, simplified optical method for measuring telomere length.
  • To establish a cost-effective and efficient alternative to existing telomere detection techniques.

Main Methods:

  • Utilized surface-enhanced Raman scattering (SERS) with DNA probes targeting telomeres and centromeres.
  • Employed magnetic nanoparticles for selective probe removal.
  • Calibrated SERS signal intensity against centromere probes for accurate telomere length assessment.

Main Results:

  • Demonstrated a direct correlation between SERS signal intensity and telomere length.
  • Validated the use of centromere probes as an internal control for reliable measurements.
  • Successfully measured telomere length without requiring polymerase chain reaction (PCR) or electrophoresis.

Conclusions:

  • The developed SERS protocol offers a simplified, rapid, and cost-effective approach for telomere length measurement.
  • This method has potential applications in diagnosing and monitoring telomere-related diseases, including cancer.
  • The protocol's ease of operation makes it a valuable tool for various research and clinical settings.