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Updated: Apr 21, 2026
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
Published on: February 9, 2012
A homodimeric BODIPY rotor as a fluorescent viscosity sensor for membrane-mimicking and cellular environments
Sangram Raut1, Joseph Kimball, Rafal Fudala
1Department of Physics and Astronomy, Texas Christian University, Fort Worth, TX 76129, USA. z.gryczynski@tcu.edu.
Abstract:
Fluorescence properties of a novel homodimeric BODIPY dye rotor for Fluorescence Lifetime Imaging Microscopy (FLIM) are reported. Steady state and time resolved fluorescence measurements established the viscosity dependent behaviour in vitro. Homodimeric BODIPY embedded in different membrane mimicking lipid vesicles (DPPC, POPC and POPC plus cholesterol) is demonstrated to be a viable sensor for fluorescence lifetime based viscosity measurements. Moreover, SKOV3 cells readily endocytosed the dye, which accumulated in membranous structures inside the cytoplasm thereby allowing viscosity mapping of internal cell components.
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