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Related Experiment Video

Updated: Apr 21, 2026

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
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Protein profiling the differences between diabetic and normal mouse cumulus cells.

Guang-Jian Jiang1, Jun-Yu Ma, Guang-Li Zhang

  • 1Diabetes Research Center, Beijing University of Chinese Medicine, Beijing, China.

Molecular Reproduction and Development
|November 11, 2014
PubMed
Summary

Type 1 diabetes impacts reproductive health by altering cumulus cell proteomes. Identifying these protein changes in diabetic mice offers potential targets for improving fertility in diabetic patients.

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Area of Science:

  • Reproductive biology
  • Endocrinology
  • Proteomics

Background:

  • Rising global incidence of diabetes, particularly in young individuals.
  • Diabetes poses significant risks to human reproductive health.
  • Understanding molecular changes in diabetic reproductive tissues is crucial.

Purpose of the Study:

  • To compare the proteomes of cumulus cells from diabetic and normal mice.
  • To identify proteins affected by type 1 diabetes in reproductive cells.
  • To explore the implications of these proteomic changes on fertility.

Main Methods:

  • Proteomic analysis of cumulus cells from super-ovulated cumulus-oocyte complexes.
  • Comparison between diabetic and normal mouse models.
  • Identification and quantification of differentially expressed proteins.

Main Results:

  • 57 proteins were up-regulated and 74 were down-regulated in diabetic cumulus cells.
  • Affected proteins are involved in cell cycle, cellular communication, epigenetics, and chromatin organization.
  • Presence of oocyte or oviductal glycoproteins in diabetic cumulus cells indicates poor follicle quality.

Conclusions:

  • Proteomic differences in cumulus cells are linked to type 1 diabetes.
  • These findings highlight molecular mechanisms affecting fertility in diabetic individuals.
  • Identified proteins represent potential therapeutic targets for enhancing reproductive health in type 1 diabetes.