Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Nonlinear KCCA in fMRI activation analysis: Self-supervised optimization and robust back-reconstruction.

Imaging neuroscience (Cambridge, Mass.)·2026

Same author

Foveated light-field compound imager.

Science advances·2026

Same author

De Novo Design of Near-Infrared Fluorescence-Activating Proteins.

Journal of the American Chemical Society·2026

Same author

Chinese herbal Formula Q attenuates letrozole-induced polycystic ovary syndrome through modulating multiple metabolic processes in female adult rats.

In silico pharmacology·2026

Same author

Comprehensive Lineage Tracing Maps the Landscape of Cell Fate Decisions in Mouse Embryogenesis.

bioRxiv : the preprint server for biology·2026

Same author

Advancing biomarker development for chronic traumatic encephalopathy: Summary and recommendations from the 2025 Leon Thal Summit.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026

Same journal

Self-Organized Nanoplasmonic Artificial Leaf for Hot-Carrier Bioelectronic Interfaces.

Nature photonics·2026

Same journal

Isotropic shrinkage of patterned vacancies enables three-dimensional nanoprecise metastructures for visible light applications.

Nature photonics·2026

Same journal

Optical convolutional spectrometer.

Nature photonics·2026

Same journal

Strong ultrafast nonlinear optical response from megaelectronvolt electrons in semiconductors.

Nature photonics·2026

Same journal

All-optical polarization control in time-varying low-index films via plasma symmetry breaking.

Nature photonics·2026

Same journal

Experimental memory control in continuous-variable optical quantum reservoir computing.

Nature photonics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 21, 2026

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Isotropic 3D Super-resolution Imaging with a Self-bending Point Spread Function.

Shu Jia¹, Joshua C Vaughan¹, Xiaowei Zhuang²

¹Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts, USA ; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA.

Nature Photonics

|November 11, 2014

Summary

This summary is machine-generated.

We developed a novel super-resolution microscopy technique using self-bending point spread functions (SB-PSFs) based on Airy beams. This method achieves precise 3D localization of fluorescent molecules for high-resolution optical imaging.

More Related Videos

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Published on: September 8, 2017

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

Related Experiment Videos

Last Updated: Apr 21, 2026

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Published on: September 8, 2017

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

Area of Science:

Optical microscopy
Super-resolution fluorescence imaging
Beam physics

Background:

Airy beams resist diffraction and bend during propagation, useful in various applications.
These properties were not previously applied to high-resolution optical microscopy.

Purpose of the Study:

To introduce a self-bending point spread function (SB-PSF) for 3D super-resolution fluorescence imaging.
To leverage Airy beam properties for enhanced molecular localization in microscopy.

Main Methods:

Designed a side-lobe-free self-bending point spread function (SB-PSF).
Implemented a two-channel detection scheme for unambiguous 3D localization.
Utilized Airy beam's non-diffracting and self-bending characteristics.

Main Results:

Achieved isotropic 3D localization precision of 10-15 nm.
Enabled imaging over a 3 μm depth with high accuracy.
Demonstrated precise localization using approximately 2000 photons per localization.

Conclusions:

The SB-PSF method offers a new approach for high-resolution 3D super-resolution microscopy.
Its unique properties enable precise molecular localization over extended imaging depths.
This technique advances optical microscopy for detailed biological structure visualization.