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Related Experiment Videos

Retinoic acid treatment of fibroblasts causes a rapid decrease in [3H]inositol uptake.

R Sinha1, K E Creek, C Silverman-Jones

  • 1Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, Bethesda, Maryland 20892.

Experimental Cell Research
|April 1, 1989
PubMed
Summary
This summary is machine-generated.

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All-trans-retinoic acid (RA) significantly reduces [3H]inositol uptake in NIH 3T3 fibroblasts by decreasing transporter Vmax. This effect is specific, reversible, and dose-dependent, impacting cellular signaling pathways.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Pharmacology

Background:

  • Inositol uptake is crucial for cellular signaling and membrane synthesis.
  • Retinoids, like all-trans-retinoic acid (RA), play vital roles in cell differentiation and proliferation.
  • Understanding how RA affects nutrient transport is key to elucidating its biological functions.

Purpose of the Study:

  • To investigate the effect of all-trans-retinoic acid (RA) on [3H]inositol uptake in NIH 3T3 fibroblasts.
  • To characterize the mechanism and specificity of RA-induced changes in inositol transport.
  • To compare the activity of RA with other retinoids and assess its impact on other nutrient uptakes.

Main Methods:

  • Treatment of NIH 3T3 fibroblasts with varying concentrations and durations of all-trans-retinoic acid (RA).

Related Experiment Videos

  • Measurement of [3H]inositol uptake kinetics, including Vmax and Kt.
  • Assessment of RA's effect on the uptake of other sugars (fucose, mannose, galactose, glucose).
  • Evaluation of RA's impact on [3H]inositol incorporation into polyphosphoinositides.

    • Main Results:

    • RA treatment caused a rapid, dose-dependent, and reversible decrease in [3H]inositol uptake, up to 70% reduction.
    • The inhibition was attributed to a decrease in Vmax, not a change in the transporter's affinity (Kt).
    • RA specifically inhibited inositol uptake, while uptake of other sugars was unaffected or enhanced; this effect was observed in specific fibroblast lines but not transformed ones.

    Conclusions:

    • All-trans-retinoic acid (RA) specifically and potently inhibits inositol uptake in 3T3 fibroblasts by reducing transporter Vmax.
    • The observed effects are reversible, dose-dependent, and occur at near-physiological RA concentrations.
    • RA's distinct effects on inositol versus other sugar uptakes highlight its specific regulatory role in fibroblast metabolism and signaling.