Bioactivity of root endophytic freshwater Hyphomycetes Anguillospora longissima (Sacc. & Syd.) Ingold

Anguillospora longissima, isolated from root as endophytic freshwater hyphomycetes, was evaluated for its bioactivity (antibacterial potential) against five bacterial strains, namely, Gram-positive (Bacillus subtilis MTCC 121) and Gram-negative (Agrobacterium tumefaciens MTCC 609, Escherichia coli MTCC 40, Erwinia chrysanthemum, and Xanthomonas pseudomonas). Antimicrobial activity was assessed by measuring the zone of inhibition with preliminary and secondary antimicrobial assays. The applied fungus was found significant for all tested bacterial strains as showen by their zone of inhibition. In preliminary antimicrobial assay, maximum zone of inhibition was recorded against Gram-negative human pathogenic bacterial strain Escherichia coli (23 mm) followed by Erwinia chrysanthemi (22 mm), Agrobacterium tumefaciens (21 mm), and Xanthomonas phaseoli (21 mm), while minimum zone of inhibition was observed against Bacillus subtilis (20 mm). In secondary antimicrobial assay, the maximum zone of inhibition was recorded against Erwinia chrysanthemi (11 mm) followed by Agrobacterium tumefaciens (10 mm), Xanthomonas phaseoli (10 mm), and Bacillus subtilis (9 mm) and minimum inhibition was found against Escherichia coli (8 mm).