Related Experiment Video
Updated: Apr 21, 2026

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
Published on: March 3, 2010
Making pyrophosphate visible: the first precipitable and real-time fluorescent sensor for pyrophosphate in aqueous
Shu-Yan Jiao1, Kun Li, Xin Wang
1Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, 610064, China. kli@scu.edu.cn xqyu@scu.edu.cn.
Abstract:
An in situ generated BINOL-DPA-Zn(ii) complex is presented as a chemo-sensing ensemble for the recognition of phosphate-based molecules. The ensemble showed high sensitivity and selectivity for pyrophosphates (PPi), and it could be successfully applied in imaging PPi in living cells. Notably, the ensemble exhibited a very low detection limit (95 nM) for PPi and could realize the real time detection of PPi by the naked eye through precipitate experiments. The ensemble also showed good selectivity towards ATP, and the selectivity coefficient for PPi and ATP was calculated to be 4.1/2.8.
Related Concept Videos
Photoluminescence: Applications
Fluorescence and Phosphorescence: Instrumentation
Variables Affecting Phosphorescence and Fluorescence

