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Updated: Apr 21, 2026
A Label-Free Segmentation Approach for Intravital Imaging of Mammary Tumor Microenvironment
Published on: May 24, 2022
Monitoring changes of tumor microenvironment in colorectal submucosa using multiphoton microscopy
Jingting Qiu1, Weizhong Jiang, Yinghong Yang
1Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, China.
Abstract:
Recently, targeting tumor microenvironment has become a novel approach for cancer therapy. Collagen is one of important components of tissue microenvironment, and has been considered as a new visible target for cancer therapy. In this work, multiphoton microscopy (MPM) was used to monitor the changes of collagen in tumor microenvironment during tumor progression. It was found that MPM facilitates imaging of tumor cells and collagen. MPM images in different tumor microenvironment during tumor progression shows obvious increase in cell number and collagen degration. In addition, the quantitative analysis of collagen content and orientation index in tumor microenvironment shows significant alteration during tumor progression. These results suggest that MPM has the ability to monitor the changes of collagen morphology in tumor microenvironment and quantify content and orientation index of collagen during tumor progression. Therefore this technique is a powerful imaging tool for the investigation of targeting tumor microenvironment for cancer therapy.
Insights
Multiphoton microscopy (MPM) visualizes collagen changes in the tumor microenvironment during cancer progression. This technique quantifies collagen degradation and alterations, aiding in developing new cancer therapies targeting the tumor microenvironment.
Area of Science:
- Oncology
- Biomedical Imaging
- Cancer Biology
Background:
- Targeting the tumor microenvironment is a novel cancer therapy approach.
- Collagen, a key component of the tumor microenvironment, is a potential therapeutic target.
- Understanding collagen's role in tumor progression is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the utility of multiphoton microscopy (MPM) for monitoring collagen changes in the tumor microenvironment during tumor progression.
- To quantitatively analyze collagen content and orientation during tumor development.
Main Methods:
- Utilized multiphoton microscopy (MPM) to image tumor cells and collagen.
- Monitored changes in the tumor microenvironment during tumor progression.
- Performed quantitative analysis of collagen content and orientation index.
Main Results:
- MPM effectively imaged tumor cells and collagen.
- Observed an increase in cell number and collagen degradation in the tumor microenvironment during progression.
- Detected significant alterations in collagen content and orientation index.
Conclusions:
- MPM is a powerful tool for monitoring collagen morphology in the tumor microenvironment.
- MPM enables quantification of collagen content and orientation during tumor progression.
- This technique supports the investigation of targeting the tumor microenvironment for cancer therapy.
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