Related Experiment Video
Updated: Apr 21, 2026

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
Published on: October 27, 2023
Targeting the proteome of cellular fractions: focus on secreted proteins
Agnieszka Latosinska1, Maria Frantzi, William Mullen
1Biotechnology Division, Biomedical Research Foundation, Academy of Athens, Soranou Ephessiou 4, 11527, Athens, Greece.
Abstract:
The high complexity of the total cellular proteome underscores the need for a more targeted investigation of particular subcellular fractions as a means to detect the changes at the level of low abundance proteins. However, this approach requires the application of an enrichment strategy. In this chapter, we present the protocols, which have been used for the analysis of secretome from cell lines, targeting the investigation of protein expression changes.
Related Concept Videos
Subcellular Fractionation
Differential Centrifugation
Differential centrifugation is...
Proteomics
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

