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The two-component system GrvRS (EtaRS) regulates ace expression in Enterococcus faecalis OG1RF.

Jung Hyeob Roh1, Kavindra V Singh2, Sabina Leanti La Rosa3

  • 1Division of Infectious Diseases, Department of Internal Medicine, The University of Texas Medical School, Houston, Texas, USA Center for the Study of Emerging and Re-emerging Pathogens, The University of Texas Medical School, Houston, Texas, USA Jung.H.Roh@uth.tmc.edu.

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A newly identified GrvRS two-component system regulates the ace gene, a key Enterococcus faecalis virulence factor. This finding explains how ace expression is controlled under various stress conditions, impacting bacterial infection.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Bacterial Pathogenesis

Background:

  • The adhesin to collagen of Enterococcus faecalis (ace) gene encodes a crucial virulence factor.
  • Ace expression is known to increase under specific conditions like serum, bile salts, urine, collagen, and elevated temperatures.
  • The regulatory mechanisms governing ace expression under these diverse conditions remain largely unelucidated.

Purpose of the Study:

  • To identify the regulatory system controlling ace expression in Enterococcus faecalis under stress conditions.
  • To investigate the role of the identified regulator in bacterial virulence, collagen binding, and biofilm formation.

Main Methods:

  • Northern hybridization to assess ace gene transcription.
  • Beta-galactosidase assays using an ace promoter-lacZ fusion.
  • Construction and analysis of a grvR deletion mutant.
  • Assessment of collagen binding and biofilm formation.
  • Evaluation of virulence in a murine urinary tract infection model.

Main Results:

  • A two-component regulatory system, GrvRS, was identified as the primary regulator of ace expression.
  • Ace transcription was virtually absent in a grvR deletion mutant under conditions that normally induce ace expression.
  • The grvR mutant exhibited reduced collagen binding and biofilm formation.
  • Virulence was attenuated in a murine urinary tract infection model for the grvR mutant.

Conclusions:

  • The GrvRS system is a major regulator of ace expression in Enterococcus faecalis.
  • GrvR plays a significant role in controlling ace expression, collagen adherence, biofilm formation, and overall virulence.
  • Understanding GrvRS regulation provides insights into Enterococcus faecalis pathogenesis.