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Updated: Apr 21, 2026
A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
Published on: November 14, 2018
A droplet microfluidic approach to single-stream nucleic acid isolation and mutation detection
Dong Jin Shin1, Yi Zhang1, Tza-Huei Wang2
1Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, USA. Address: 122 Clark Hall, 3400 N Charles St, Baltimore, Maryland, USA.
Abstract:
In this work, a droplet microfluidic platform for genetic mutation detection from crude biosample is described. Single-stream integration of nucleic acid isolation and amplification is realized on a simple fluidic cartridge. Subsequent DNA melting curve is employed with signal normalizing algorithm to differentiate heterozygous K-ras codon 12 c.25G>A mutant from the wildtype. This technique showcases an alternative to modular bench-top approaches for genetic mutation screening, which is of interest to decentralized diagnostic platforms.
