A new PANI biosensor based on catalase for cyanide determination

Area of Science:

• Environmental Science
• Analytical Chemistry
• Materials Science

Background:

• Cyanide compounds pose significant environmental and health risks, necessitating accurate detection methods.
• Environmental analysis frequently involves measuring widespread cyanide compounds.
• Developing sensitive and selective sensors is crucial for monitoring cyanide levels.

Purpose of the Study:

• To develop a highly sensitive, reliable, and selective amperometric sensor for cyanide determination.
• To utilize a polyaniline conductive polymer matrix for enzyme immobilization.
• To optimize and characterize the performance of the developed cyanide biosensor.

Main Methods:

• Immobilization of the enzyme catalase onto a polyaniline conductive polymer via electropolymerization.
• Control and characterization of immobilization steps using electrochemical impedance spectroscopy.
• Optimization of parameters including pH, temperature, aniline concentration, enzyme concentration, and electropolymerization scans.
• Determination of cyanide in artificial wastewater samples.
• Analysis of biosensor characteristics such as reproducibility and storage stability.

Main Results:

• A highly sensitive, reliable, and selective amperometric sensor for cyanide detection was successfully developed.
• Electropolymerization enabled effective immobilization of catalase.
• Optimal conditions for sensor performance were identified.
• The sensor demonstrated effectiveness in determining cyanide in artificial wastewater samples.
• The biosensor exhibited good reproducibility and storage stability.

Conclusions:

• The developed polyaniline-based amperometric biosensor offers a sensitive and reliable method for cyanide detection.
• The sensor is suitable for environmental monitoring, particularly for analyzing cyanide in wastewater.
• Electrochemical immobilization techniques provide a robust platform for biosensor development.