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Updated: Apr 21, 2026

Polysome Profiling in Leishmania, Human Cells and Mouse Testis
Published on: April 8, 2018
Separation of basic proteins from Leishmania using a combination of Free flow electrophoresis (FFE) and 2D
Marie-Christine Brotherton1, Gina Racine, Marc Ouellette
1Centre de Recherche en Infectiologie, CHU-Québec research centre, 2705 boul Laurier, Québec, Canada, G1V 4G2.
Abstract:
Basic proteins, an important class of proteins in intracellular organisms such as Leishmania, are usually underrepresented on 2D gels. This chapter describes a method combining basic proteins fractionation using Free flow electrophoresis in isoelectric focusing mode (IEF-FFE) followed by protein separation using two-dimensional gel electrophoresis (2-DE) in basic conditions. The combination of these two techniques represents a great improvement for the visualization of Leishmania proteins with basic pI using 2D gels.
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