Measurement of nitric oxide in mast cells with the fluorescent indicator DAF-FM diacetate
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Updated: Apr 21, 2026
En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
Published on: February 25, 2016
Chris D St Laurent1, Tae Chul Moon, A Dean Befus
1Pulmonary Research Group, Department of Medicine, University of Alberta, 116th Street & 87 Avenue, Edmonton, AB, Canada, T6G 2R3.
Abstract:
The production of nitric oxide in mast cells has been difficult to measure due to the low amounts made by mast cells, as well as limitations in the specificity and sensitivity of the assays available. We present here a sensitive and specific 96-well plate-based method to directly measure NO using the cell-permeable fluorescent compound DAF-FM diacetate.
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