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Adult Stem Cells

Adult Stem Cells

Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...

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Measuring Single-Cell Aging with an Imaging-based Biomarker of Chromatin and Epigenetic Aging

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Measuring the aging process in stem cells.

Yi Liu¹, Gary Van Zant, Ying Liang

¹Division of Hematology/Bone Marrow Transplantation, Department of Internal Medicine, Markey Cancer Center, University of Kentucky, 800 Rose Street, Lexington, KY, 40536, USA.

Methods in Molecular Biology (Clifton, N.J.)

|November 13, 2014

Summary

This summary is machine-generated.

Hematopoietic stem cells (HSCs) maintain blood cell production throughout life. Aging alters HSC composition and function, which can be studied using flow cytometry and fluorescence-activated cell sorting in mouse models.

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Area of Science:

Stem Cell Biology
Hematopoiesis
Aging Research

Background:

Stem cells continuously replenish tissues through self-renewal and differentiation.
Hematopoietic stem cells (HSCs) are crucial for lifelong blood cell production.
HSCs are heterogeneous, with subsets biased towards lymphoid or myeloid lineages.

Purpose of the Study:

To investigate age-associated changes in HSC phenotype and function.
To characterize the alterations in HSC pool composition during aging.
To establish methods for identifying and analyzing HSC aging.

Main Methods:

Utilized flow cytometry and fluorescence-activated cell sorting (FACS) for HSC analysis.
Employed a mouse model for studying HSC aging.
Isolated bone marrow cells from young and old mice, stained with specific antibodies.

Main Results:

Identified HSCs based on negative lineage marker expression and positive stem cell markers.
Distinguished lineage-biased HSCs using SLAM/CD150 expression and Hoechst efflux.
Demonstrated that HSC pool composition changes with age.

Conclusions:

HSCs undergo significant age-related changes in their cellular and molecular characteristics.
FACS is a powerful tool for isolating and studying HSC aging mechanisms.
Understanding HSC aging is critical for maintaining lifelong hematopoiesis.