Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Local oxygen tension dictates hematopoietic cell growth and potency.

Leukemia·2026
Same author

Performance of rapid antigen tests to detect SARS-CoV-2 variant diversity and correlation with viral culture positivity: implication for diagnostic development and future public health strategies.

mBio·2024
Same author

Mitigation of supply chain challenges in cell therapy manufacturing: perspectives from the cord blood alliance.

Stem cells translational medicine·2024
Same author

Non-viral expression of chimeric antigen receptors with multiplex gene editing in primary T cells.

Frontiers in bioengineering and biotechnology·2024
Same author

The fulfilled promise and unmet potential of umbilical cord blood.

Current opinion in hematology·2024
Same author

Insights into highly engraftable hematopoietic cells from 27-year cryopreserved umbilical cord blood.

Cell reports. Medicine·2023

Related Experiment Video

Updated: Apr 21, 2026

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis
08:27

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Published on: July 24, 2014

13.0K

Culturing protocols for human multipotent adult stem cells.

Bart Vaes1, Sara Walbers, Kristel Gijbels

  • 1ReGenesys BVBA, Heverlee, Belgium.

Methods in Molecular Biology (Clifton, N.J.)
|November 13, 2014
PubMed
Summary

This study details methods for culturing multipotent adult progenitor cells (MAPCs) from human bone marrow. These procedures enable controlled expansion of clinical-grade MAPCs for advanced clinical trials.

More Related Videos

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
07:33

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

Published on: August 3, 2015

8.4K
A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
07:14

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

Published on: May 17, 2021

5.6K

Related Experiment Videos

Last Updated: Apr 21, 2026

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis
08:27

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Published on: July 24, 2014

13.0K
A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
07:33

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

Published on: August 3, 2015

8.4K
A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
07:14

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

Published on: May 17, 2021

5.6K

Area of Science:

  • Stem cell biology
  • Cell culture technology
  • Regenerative medicine

Background:

  • Human bone marrow contains multipotent mesenchymal stromal cells (MSCs).
  • A subset of MSCs, multipotent adult progenitor cells (MAPCs), possess unique primitive properties.
  • Expansion of MAPCs to clinically relevant numbers is challenging.

Purpose of the Study:

  • To present culture procedures for initiating and expanding human MAPCs.
  • To describe characterization assays for maintaining MAPC properties.
  • To facilitate the production of clinical-grade MAPCs for therapeutic applications.

Main Methods:

  • Isolation of MAPCs from human bone marrow.
  • Optimization of cell culture conditions for controlled expansion.
  • Development of assays to monitor MAPC identity and purity.
  • Utilizing cell culturing platforms for scalable production.

Main Results:

  • Established reproducible culture procedures for MAPC initiation and expansion.
  • Demonstrated maintenance of primitive MAPC characteristics during culture.
  • Achieved expansion of MAPCs to levels suitable for clinical testing.

Conclusions:

  • Culture methods support the controlled expansion of human MAPCs.
  • Characterization assays ensure the quality of clinical-grade MAPC products.
  • These advancements enable the progression of MAPC-based therapies in clinical trials.