Closing the MCM cycle at replication termination sites
Related Experiment Video
Updated: Apr 21, 2026

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
Published on: July 26, 2024
Armelle Lengronne1, Philippe Pasero1
1Institute of Human Genetics CNRS, Montpellier, France.
Abstract:
The initiation of eukaryotic DNA replication is a highly regulated process conserved from yeast to human. The past decade has seen significant advances in understanding how the CMG (Cdc45‐MCM‐GINS) replicative helicase is loaded onto DNA. However, very little was known on how this complex is removed from chromatin at the end of S phase. Two papers in a recent issue of Science [1], [2] show that in yeast and in Xenopus, the CMG complex is unloaded at replication termination sites by an active mechanism involving the polyubiquitylation of Mcm7.
Related Concept Videos
Restarting Stalled Replication Forks
Restarting Stalled Replication Forks
Separation of Sister Chromatids
At the onset of anaphase, separase, a proteolytic enzyme, is...
DNA Damage can Stall the Cell Cycle
DNA Damage Can Stall the Cell Cycle
The Spindle Assembly Checkpoint
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...

