Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.6K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.6K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

2.5K
2.5K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

4.8K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
4.8K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

10.4K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.4K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

3.4K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.4K
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

4.0K
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
4.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Condensin and topoisomerases cooperate to relieve topological stress at stalled replication forks.

Nature communications·2026
Same author

ATM lifts the cGAS handbrake on DNA replication.

Nature cell biology·2026
Same author

Slx4 and Fun30/SMARCAD1 coordinate S-phase checkpoint regulation and replication fork protection in response to Top1-DNA crosslinks.

Nucleic acids research·2026
Same author

Transcription-Replication Conflicts and Incomplete Replication as a Cause of Micronuclei-Driven Chromoanagenesis.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Synthetic Lethal Combinations of DNA Repair Inhibitors and Genotoxic Agents to Target High-Risk Diffuse Large B Cell Lymphoma.

Hematological oncology·2025
Same author

RNA Pol II-based regulations of chromosome folding.

Cell genomics·2025

Related Experiment Video

Updated: Apr 21, 2026

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

1.8K

Closing the MCM cycle at replication termination sites.

Armelle Lengronne1, Philippe Pasero1

  • 1Institute of Human Genetics CNRS, Montpellier, France.

EMBO Reports
|November 14, 2014
PubMed
Summary

Researchers discovered how the CMG helicase complex is removed from DNA after replication. This process involves polyubiquitylation of Mcm7, ensuring proper termination of DNA replication in eukaryotes.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Eukaryotic DNA replication initiation is tightly regulated.
  • Significant progress has been made in understanding CMG (Cdc45-MCM-GINS) replicative helicase loading.
  • Mechanisms for CMG complex removal from chromatin post-S phase were largely unknown.

Purpose of the Study:

  • To elucidate the mechanism of CMG complex removal from chromatin at the end of S phase.
  • To identify the factors and processes involved in DNA replication termination.

Main Methods:

  • Studies were conducted in yeast and Xenopus models.
  • Investigation focused on replication termination sites.
  • Analysis involved examining the polyubiquitylation of Mcm7.

More Related Videos

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
07:37

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Published on: September 27, 2024

2.7K
Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

11.4K

Related Experiment Videos

Last Updated: Apr 21, 2026

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

1.8K
Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
07:37

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Published on: September 27, 2024

2.7K
Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

11.4K

Main Results:

  • The CMG complex is actively unloaded from chromatin at replication termination sites.
  • Polyubiquitylation of the Mcm7 subunit is a key event in CMG unloading.
  • This mechanism is conserved in both yeast and Xenopus.

Conclusions:

  • CMG helicase removal is an active, regulated process.
  • Mcm7 polyubiquitylation drives CMG unloading, completing DNA replication.
  • Findings provide crucial insights into the termination phase of eukaryotic DNA replication.