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Antimicrobial susceptibility patterns of enterobacteriaceae isolated from HIV-infected patients in Kinshasa
Jean-Marie Liesse Iyamba1, José Mulwahali Wambale1, Ntondo Za Balega Takaisi-Kikuni1
1Laboratory of Experimental and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo.
Introduction:
People infected by Human Immunodeficiency Virus (HIV) are susceptible to develop severe bacterial infections. We set out to determine the frequency and the sensitivity to antibiotics of enterobaceriaceae isolated from urine and feces of HIV-infected persons.
Methods:
Urine and feces samples were collected from HIV-infected patients of the Centre de Traitement Ambulatoire de Kabinda (CTA/Kabinda, Kinshasa) and analyzed at the Reference National Laboratory for HIV/AIDS and Sexually Transmitted Infections. The isolated enterobacteriaceae strains were identified by conventional microbiological methods. Antibiotic sensitivity pattern was carried out by disc diffusion method.
Results:
THE FOLLOWING BACTERIA PATHOGENS WERE ISOLATED: Escherichia coli, Klebsiella, Enterobacter, Proteus, and Providencia. Most species were sensitive to cefotaxim, ceftriaxon, and gentamicin and resistant to chloramphenicol, cotrimoxazole, tetracycline, and norfloxacin.
Conclusion:
The results of the present study show that the most frequently bacteria isolated were Esherichia coli and cefotaxim, ceftriaxon, and gentamicin were the most active antibiotics.
Insights
Human Immunodeficiency Virus (HIV)-infected individuals frequently harbor Enterobacteriaceae in urine and feces. Cefotaxim, ceftriaxone, and gentamicin demonstrated the highest effectiveness against these common bacterial pathogens.
Area of Science:
- Medical Microbiology
- Infectious Diseases
- Public Health
Background:
- Human Immunodeficiency Virus (HIV) infection compromises the immune system, increasing susceptibility to severe bacterial infections.
- Enterobacteriaceae are common causes of infections, particularly in immunocompromised populations.
Purpose of the Study:
- To determine the frequency of Enterobacteriaceae isolated from urine and feces of HIV-infected individuals.
- To assess the antibiotic sensitivity patterns of these isolated Enterobacteriaceae strains.
Main Methods:
- Urine and fecal samples were collected from HIV-infected patients.
- Enterobacteriaceae were identified using conventional microbiological techniques.
- Antibiotic susceptibility testing was performed via the disc diffusion method.
Main Results:
- Key bacterial pathogens identified included Escherichia coli, Klebsiella, Enterobacter, Proteus, and Providencia.
- The majority of isolates were sensitive to cefotaxime, ceftriaxone, and gentamicin.
- Resistance was noted against chloramphenicol, cotrimoxazole, tetracycline, and norfloxacin.
Conclusions:
- Escherichia coli was the most frequently isolated bacterium.
- Cefotaxime, ceftriaxone, and gentamicin were identified as the most effective antibiotics against the studied Enterobacteriaceae.
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