Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

5.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.5K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

6.4K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
6.4K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

2.1K
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tumor microenvironment-mediated interactions between macrophages and cancer cells define immunoregulatory transcriptional programs.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Personalized chemotherapy guidance using patient-derived scaffolds from peritoneal colorectal metastases.

Experimental hematology & oncology·2026
Same author

SiMSen-Seq STR sequencing across five laboratories enables lowered noise thresholds and improved allele calling.

Forensic science international. Genetics·2026
Same author

A single-cell multi-omics assay for simultaneous measurement of vector copy number and protein expression in CAR T cells.

Molecular therapy. Advances·2026
Same author

Understanding the qPCR Standard Curve: From Assay Validation to Absolute Quantification and Variance PCR.

International journal of molecular sciences·2026
Same author

Ageing promotes metastasis via activation of the integrated stress response.

Nature·2026

Related Experiment Video

Updated: Apr 21, 2026

Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts
07:18

Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts

Published on: June 13, 2019

7.5K

Normal and functional TP53 in genetically stable myxoid/round cell liposarcoma.

Anders Ståhlberg1, Christina Kåbjörn Gustafsson1, Katarina Engtröm2

  • 1Sahlgrenska Cancer Center, Department of Pathology, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden.

Plos One
|November 14, 2014
PubMed
Summary

This study found that Myxoid/round-cell liposarcoma (MLS/RCLS) typically retains functional TP53 genes, with no observed mutations or significant alterations impacting its response to DNA damage. This supports the low rate of secondary mutations in MLS/RCLS.

More Related Videos

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

10.2K
Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells
09:37

Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells

Published on: August 25, 2021

2.3K

Related Experiment Videos

Last Updated: Apr 21, 2026

Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts
07:18

Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts

Published on: June 13, 2019

7.5K
Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

10.2K
Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells
09:37

Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells

Published on: August 25, 2021

2.3K

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Myxoid/round-cell liposarcoma (MLS/RCLS) is a soft tissue sarcoma often driven by FUS-DDIT3 or EWSR1-DDIT3 fusion genes.
  • The role and status of the TP53 tumor suppressor gene in MLS/RCLS remain unclear, with conflicting reports in existing literature.

Purpose of the Study:

  • To investigate the mutation status, expression, and functional activity of TP53 in MLS/RCLS cell lines.
  • To determine if the FUS-DDIT3 fusion protein influences TP53 responses to DNA damage.

Main Methods:

  • Analysis of TP53 mutations, expression, and posttranslational modifications in four MLS/RCLS cell lines.
  • Irradiation experiments to assess TP53 activation and P21 induction.
  • Transfection studies to evaluate FUS-DDIT3 impact on TP53.
  • Next-generation sequencing (Ion Torrent AmpliSeq Cancer Hotspot panel) for mutation screening.

Main Results:

  • Three MLS/RCLS cell lines expressed normal TP53 proteins.
  • Irradiation induced typical TP53 posttranslational modifications and P21 expression in two cell lines.
  • The FUS-DDIT3 fusion protein did not affect irradiation-induced TP53 responses.
  • No disease-associated TP53 mutations or dysfunctional alleles were detected via sequencing.

Conclusions:

  • The findings suggest that functional TP53 genes are common in MLS/RCLS.
  • This functional TP53 status is consistent with the generally low incidence of secondary genetic mutations observed in this tumor type.
  • MLS/RCLS may not frequently rely on TP53 inactivation for tumorigenesis.