Localization of tobacco germin-like protein 1 in leaf intercellular space

To characterize leaf cell wall proteins relating the architectural changes of leaves, we analyzed Nicotiana tabacum leaf cell wall proteins that were extracted by the treatment with lithium chloride. Some of these proteins showed amino acid sequence homology to some germin-like proteins (GLP). Based of those sequences, we isolated the cDNA encoding the GLPs (NtGLP1-1, NtGLP2-1). Phylogenetic analysis including de novo assembled tobacco GLPs using EST clones, revealed that tobacco GLPs belong to at least 5 different subgroups of GLP and both NtGLP1 and NtGLP2 belong to GLP subfamily 3. We showed that the NtGLP1 actually localizes to cell wall and revealed that it predominantly localized at specific sites on the leaf cell wall where intercellular attachment was just bifurcated. Expression of the NtGLP1 mRNA was mainly detected in leaves especially at elongating stage. NtGLP1 is possibly relevant to development of leaf intercellular space.