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Resistance to foot-and-mouth disease virus mediated by trans-acting cellular products
J C de la Torre1, S de la Luna, J Diez
1Centro de Biologia Molecular, Universidad Autónoma, Madrid, Spain.
Abstract:
Upon serial passage of BHK-21 cells persistently infected with foot-and-mouth disease virus (FMDV) C-S8c1, cells with increased resistance to the virus were selected (J. C. de la Torre, E. Martinez-Salas, J. Diez, and E. Domingo, J. Virol. 63:59-63, 1989). Two highly resistant cell clones, 74A11 and 74D12, were transformed to puromycin resistance (Purr) and were fused to BHK-21 cells transformed to neomycin resistance (Neor). The hybrid Neor Purr cells showed the specific resistance to FMDV C-S8c1 characteristic of clones 74A11 and 74D12. The results suggest that resistance to FMDV C-S8c1 is mediated by trans-acting cellular products. The possibility of engineering constitutive resistance to FMDV is discussed.
Insights
Researchers developed cell lines resistant to foot-and-mouth disease virus (FMDV). Hybrid cells combining resistance traits inherited specific FMDV resistance, suggesting cellular products mediate this immunity.
Area of Science:
- Virology
- Cell Biology
- Immunology
Background:
- Foot-and-mouth disease virus (FMDV) poses a significant threat to livestock.
- Developing cell lines with inherent resistance is crucial for FMDV research and control.
- Previous work identified FMDV-resistant cell clones (74A11 and 74D12) after serial passage.
Purpose of the Study:
- To investigate the genetic basis of FMDV resistance in selected cell clones.
- To determine if FMDV resistance can be transferred and maintained in hybrid cells.
- To explore the potential for engineering constitutive FMDV resistance.
Main Methods:
- Serial passage of BHK-21 cells with FMDV C-S8c1 to select resistant clones.
- Transformation of resistant clones (74A11, 74D12) with puromycin resistance (Purr).
- Fusion of Purr-transformed cells with neomycin resistance (Neor) transformed BHK-21 cells to create hybrid cells.
Main Results:
- Hybrid Neor Purr cells exhibited specific resistance to FMDV C-S8c1, mirroring the parental resistant clones.
- The transfer of resistance traits to hybrid cells was successful.
- These findings indicate that cellular factors, not viral components, are responsible for resistance.
Conclusions:
- FMDV C-S8c1 resistance is mediated by trans-acting cellular products.
- The study supports the feasibility of engineering constitutive resistance to FMDV.
- Further research into these cellular factors could lead to novel antiviral strategies.