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Draft Genome Sequence of an Extensively Drug-Resistant Acinetobacter baumannii Indigo-Pigmented Strain

German Traglia1, Elisabet Vilacoba1, Marisa Almuzara2

  • 1Instituto de Microbiología y Parasitología Médica (IMPaM, UBA-CONICET), Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.

Genome Announcements
|November 15, 2014
PubMed

Insights

An outbreak of extensively drug-resistant Acinetobacter baumannii in Argentina was investigated. The genome of a key indigo-pigmented strain was sequenced, revealing genetic elements linked to antibiotic resistance and spread.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Acinetobacter baumannii is a significant nosocomial pathogen.
  • An outbreak of indigo-pigmented A. baumannii occurred in Buenos Aires in 2013.
  • This pathogen is often associated with multidrug resistance.

Purpose of the Study:

  • To present the draft genome sequence of an extensively drug-resistant Acinetobacter baumannii strain (A. baumannii A33405) involved in a 2013 hospital outbreak in Buenos Aires, Argentina.
  • To identify genetic elements contributing to the strain's resistance profile and potential for horizontal gene transfer.

Main Methods:

  • Whole-genome sequencing of Acinetobacter baumannii A33405.
  • Bioinformatic analysis to identify genes and genetic elements.
  • Antimicrobial susceptibility testing (implied by XDR classification).

Main Results:

  • The draft genome sequence of A. baumannii A33405 was obtained.
  • The strain was confirmed as extensively drug-resistant (XDR).
  • Genetic elements associated with horizontal genetic transfer and multiple antibiotic resistances were identified within the genome.

Conclusions:

  • The genome sequencing provides insights into the genetic basis of the extensively drug-resistant Acinetobacter baumannii outbreak strain.
  • Understanding these genetic elements is crucial for developing strategies to control the spread of resistant bacteria in healthcare settings.

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