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Updated: Apr 21, 2026

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
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Genome Sequence of the Anterograde-Spread-Defective Herpes Simplex Virus 1 Strain MacIntyre

Moriah L Szpara1, Yolanda R Tafuri2, Lance Parsons3

  • 1moriah@psu.edu.

Genome Announcements
|November 15, 2014
PubMed

Insights

Researchers sequenced the herpes simplex virus 1 (HSV-1) MacIntyre strain from a lethal encephalitis case. This unique strain shows limited neuronal spread, moving only in the retrograde direction.

Area of Science:

  • Virology
  • Genomics
  • Neuroscience

Background:

  • Herpes simplex virus 1 (HSV-1) is a common human pathogen.
  • HSV encephalitis is a severe neurological condition with high mortality.
  • Understanding viral strains like MacIntyre is crucial for studying viral pathogenesis and spread.

Purpose of the Study:

  • To determine the complete genome sequence of the HSV-1 MacIntyre strain.
  • To investigate the unique neuronal spread characteristics of the MacIntyre strain.

Main Methods:

  • Paired-end Illumina deep sequencing was employed.
  • De novo assembly was used to reconstruct the viral genome.

Main Results:

  • The complete genome sequence of the HSV-1 MacIntyre strain was determined.
  • The MacIntyre strain exhibits a unique limitation in neuronal spread, exclusively moving in the retrograde direction.

Conclusions:

  • The genome sequencing provides a basis for understanding the MacIntyre strain's unique properties.
  • The observed retrograde-only neuronal spread offers insights into HSV-1 neurotropism and pathogenesis.

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