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Antigen-driven immunoglobulin production by human colostral lymphocytes
1Department of Pediatrics, C.S. Mott Children's Hospital, University of Michigan Medical School, Ann Arbor 48109.
Pediatric Research
|March 1, 1989
Summary
Human milk contains immune cells that may protect newborns. This study shows colostral B-lymphocytes can produce antibodies against polio virus, suggesting a role in infant immunity.
Area of Science:
- Immunology
- Neonatal Health
- Lactation Biology
Background:
- Human milk is rich in secretory IgA, produced by plasma cells near ductal epithelium.
- The presence of lymphocytes, granulocytes, and macrophages in milk suggests potential protective functions for neonates.
- The specific role of milk B-lymphocytes in infant immunity has been debated.
Purpose of the Study:
- To investigate the functionality of B-lymphocytes found in human milk.
- To determine if colostral B-lymphocytes can produce antibodies upon stimulation.
- To explore the potential of milk-borne immune cells in protecting the suckling neonate.
Main Methods:
- A novel technique for separating milk cells before culture was employed.
- Colostral B-lymphocytes were isolated and cultured.
- Cells were stimulated with a vaccine strain of poliovirus to assess immunoglobulin production.
Main Results:
- The study successfully separated and cultured B-lymphocytes from colostrum.
- Colostral B-lymphocytes demonstrated the ability to elaborate immunoglobulin.
- This immunoglobulin production occurred in response to antigenic stimulation by poliovirus.
Conclusions:
- B-lymphocytes present in human colostrum are functional.
- These B-lymphocytes can produce antibodies, specifically immunoglobulin, when exposed to antigens like poliovirus.
- This finding supports the hypothesis that milk-borne immune cells contribute to the protection of the suckling neonate against infection.