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[Oncogene of human papillomavirus]
1Dept. of Tumor Virology, Research Institute for Microbial Diseases, Osaka University.
Gan to Kagaku Ryoho. Cancer & Chemotherapy
|March 1, 1989
Summary
Specific human papillomavirus (HPV) types are strongly linked to cervical cancer development. HPV-16
Area of Science:
- Oncology
- Virology
- Molecular Biology
Context:
- Cervical cancer is a significant global health concern.
- The role of human papillomavirus (HPV) in cervical carcinogenesis has been investigated.
- Previous studies suggested an association between certain HPV types and cervical cancer.
Purpose:
- To definitively establish HPV as a causative agent of cervical cancer.
- To identify specific HPV genes responsible for cellular transformation and tumor development.
- To elucidate the mechanisms underlying HPV-induced cervical carcinogenesis.
Summary:
- Cellular DNA from cervical carcinomas induced NIH 3T3 cell transformation, retaining HPV sequences.
- HPV-16 demonstrated transforming activity in established mouse and rat cells via its E6 and E7 open reading frames (ORFs).
- The E6 ORF influenced tumorigenicity, while the E7 ORF affected cell growth, saturation density, soft agar colony formation, and immortalization.
- HPV alone may not be sufficient, as primary rat cells required a latency period for transformation by HPV-16 E6/E7 ORFs, highlighting the importance of studying changes during this phase.
Impact:
- Provides strong evidence implicating specific HPV types in cervical cancer etiology.
- Identifies the E6 and E7 ORFs of HPV-16 as key oncogenes driving cellular transformation.
- Suggests that additional cellular events or co-factors are necessary for complete cervical carcinogenesis.
- Underscores the need for further research into the latency period of HPV-induced transformation to fully understand cervical cancer development.