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Coconut (Cocos nucifera l.) pollen cryopreservation.
A Karun1, K K Sjini2, V Niral1
1Central Plantation Crops Research Institute, Kasaragod, Kerala, India.
Cryo Letters
|November 15, 2014
Summary
Cryopreservation effectively conserves coconut (Cocos nucifera) pollen, maintaining germination and fertility for long-term germplasm storage. This method enables the establishment of vital pollen cryobanks for coconut genetic resource conservation.
Area of Science:
- Plant genetics
- Cryobiology
- Conservation biology
Background:
- Coconut genetic resources face threats from pests, pathogens, and environmental changes.
- Cryopreservation offers a safe, cost-effective method for long-term conservation of recalcitrant seeds like coconut.
Purpose of the Study:
- To evaluate the impact of cryopreservation on coconut pollen germination and fertility.
- To assess the effect of cryostorage duration on pollen viability.
Main Methods:
- Pollen from two coconut varieties (West Coast Tall and Chowghat Orange Dwarf) was collected over three years.
- Pollen was desiccated to 7.5% moisture content and cryopreserved via direct immersion in liquid nitrogen.
Main Results:
- Cryopreserved pollen showed comparable germination and pollen tube length to desiccated pollen across varieties and harvest years.
- Pollen germination remained stable for up to 6 years of cryostorage for West Coast Tall.
- Normal seed set was achieved using pollen cryostored for up to 4 years, with cryopreserved pollen generally exhibiting higher vigor than non-cryopreserved samples.
Conclusions:
- Successful cryopreservation of coconut pollen is demonstrated.
- The establishment of pollen cryobanks for long-term coconut germplasm conservation is now feasible.
