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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Genetic Screens02:46

Genetic Screens

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Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Updated: Apr 20, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
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Adapting CRISPR/Cas9 for functional genomics screens.

Abba Malina1, Alexandra Katigbak1, Regina Cencic1

  • 1Department of Biochemistry, McGill University, Montreal, Quebec, Canada.

Methods in Enzymology
|November 16, 2014
PubMed
Summary
This summary is machine-generated.

CRISPR/Cas9 genome editing is a versatile tool for genetic modification. Retrovirus vectors enable stable delivery of CRISPR components for advanced functional genomics and in vivo screens.

Keywords:
CRISPRCas9Functional genomicsGenetic screeningRetroviral vectors

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • CRISPR/Cas9 is a revolutionary genome editing technology.
  • Its ease of use and speed enable modifications in diverse cell types and organisms.
  • Retrovirus-based systems offer stable expression of CRISPR components.

Purpose of the Study:

  • To describe methods for designing and generating single guide (sg) RNA libraries.
  • To highlight considerations for using all-in-one retroviral vectors for CRISPR applications.
  • To facilitate large-scale functional genomics and in vivo genetic screens.

Main Methods:

  • Development of retrovirus-based expression platforms.
  • Simultaneous delivery of Cas9 nuclease and sgRNAs.
  • Design and generation of sgRNA libraries in retroviral vectors.

Main Results:

  • Stable and reproducible expression of CRISPR/Cas9 editing tools.
  • Broad cell targeting spectrum compatible with in vivo screens.
  • Facilitation of customizable genetic modifications and functional genomics.

Conclusions:

  • Retroviral vectors are effective for delivering CRISPR/Cas9 components.
  • sgRNA library design in these vectors supports advanced genetic screening.
  • This approach enhances the utility of CRISPR for research and therapeutic development.