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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Related Experiment Video

Updated: Apr 20, 2026

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish
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Cas9-based genome editing in zebrafish.

Andrew P W Gonzales1, Jing-Ruey Joanna Yeh1

  • 1Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, USA; Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA.

Methods in Enzymology
|November 16, 2014
PubMed
Summary
This summary is machine-generated.

CRISPR/Cas9 genome editing in zebrafish offers powerful tools for genetic manipulation. This technology enables precise DNA alterations, gene knock-outs, and the creation of disease models, advancing functional genomics research.

Keywords:
CRISPRCas9Chromosomal conversionChromosomal deletionGene-editingGenome engineeringHomology-directed repairKnock-inKnock-outTargeted integrationTargeted mutagenesisZebrafish

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Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • The Cas9 endonuclease from Streptococcus pyogenes is a versatile genome editing tool.
  • CRISPR/Cas9 technology has been successfully applied across diverse biological systems.

Purpose of the Study:

  • To review the origin and applications of CRISPR/Cas9 genome editing in zebrafish.
  • To highlight the design and implementation of Cas9-based genome engineering in zebrafish models.

Main Methods:

  • Utilizing synthetic guide RNAs to direct Cas9 to specific genomic loci in zebrafish.
  • Employing Cas9 for various genetic modifications including knock-outs and targeted DNA integration.

Main Results:

  • Cas9 enables precise sequence alterations, chromosomal deletions, and multi-gene disruptions in zebrafish.
  • Efficient generation of knock-outs, conditional alleles, tagged proteins, and reporter lines is achievable.

Conclusions:

  • Cas9-based genome editing significantly advances zebrafish functional genomics and disease modeling.
  • The ease and efficiency of Cas9 technology open new avenues for genetic engineering in zebrafish.