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A marker of acid-secreting membrane movement in rat gastric parietal cells

F Mercier1, H Reggio, G Devilliers

  • 1Centre CNRS-INSERM de Pharmacologie-Endocrinologie, Montpellier, France.

Biology of the Cell
|January 1, 1989
PubMed

Insights

A new antibody (mab 146.14) identifies a 95-kD protein in rat gastric parietal cells. This protein marks acid-secreting membranes, moving from the cytoplasm to the cell surface upon stimulation.

Area of Science:

  • Cell Biology
  • Gastroenterology
  • Immunology

Background:

  • Rat gastric parietal cells secrete acid via the H+ -K+ ATPase proton pump.
  • Understanding the dynamic movement of these acid-secreting membranes is crucial for comprehending gastric acid regulation.

Purpose of the Study:

  • To produce and characterize a monoclonal antibody (mab 146.14) that serves as a marker for acid-secreting membranes in rat gastric parietal cells.
  • To investigate the protein composition and localization of the antigen recognized by mab 146.14.

Main Methods:

  • Production and characterization of monoclonal antibody mab 146.14.
  • Biochemical analysis of purified membrane fractions from rat gastric mucosa.
  • Immunocytochemical localization studies using light and electron microscopy.

Main Results:

  • Mab 146.14 recognized a 95-kD protein component in rat gastric membranes, similar to H+ -K+ ATPase-enriched membranes.
  • The antigen's density shifted between resting and stimulated tissues, indicating dynamic changes.
  • Immunocytochemistry revealed the 95-kD antigen is exclusively on acid-secreting membranes in parietal cells, translocating from cytoplasmic tubulovesicles to apical microvilli upon stimulation.

Conclusions:

  • The 95-kD antigen, likely part of the rat gastric proton pump, is a reliable marker for acid-secreting membranes.
  • The observed translocation supports a membrane fusion model for acid secretion, involving cytoplasmic storage pools and apical insertion.
  • Mab 146.14 provides a valuable tool for studying the regulation of gastric acid secretion.

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