Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

One-step synthesis of biocompatible magnetite/silk fibroin core-shell nanoparticles.

Journal of materials chemistry. B·2020
Same author

Diagnostic accuracy of the UBC<sup>®</sup> Rapid Test for bladder cancer: A meta-analysis.

Oncology letters·2018
Same author

The preliminary application of liver imaging reporting and data system (LI-RADS) with contrast-enhanced ultrasound (CEUS) on small hepatic nodules (≤ 2cm).

Journal of Cancer·2018
Same author

Fistula formation between the arterial blood supply of pulmonary sequestration and the oesophagus accompanied by an intermittent haematemesis.

Interactive cardiovascular and thoracic surgery·2018
Same author

[Effect of ulinastatin on perioperative glycocalyx and lung function in patients undergoing mitral valve replacement surgery].

Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences·2018
Same author

Event-Based Communication and Finite-Time Consensus Control of Mobile Sensor Networks for Environmental Monitoring.

Sensors (Basel, Switzerland)·2018

Related Experiment Video

Updated: Apr 20, 2026

Synthesis of Graphene-Hydroxyapatite Nanocomposites for Potential Use in Bone Tissue Engineering
07:14

Synthesis of Graphene-Hydroxyapatite Nanocomposites for Potential Use in Bone Tissue Engineering

Published on: July 27, 2022

4.3K

Osteoinductive-nanoscaled silk/HA composite scaffolds for bone tissue engineering application.

Xiaowei Huang1, Shumeng Bai1, Qiang Lu1

  • 1National Engineering Laboratory for Modern Silk & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou, 215123, People's Republic of China.

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
|November 18, 2014
PubMed
Summary

Silk/hydroxyapatite (HA) nanoparticles enhance bone regeneration scaffolds. These nano-enhanced scaffolds improve cell growth and bone formation, offering a superior microenvironment for bone repair compared to traditional composite scaffolds.

Keywords:
bone tissue engineeringhydroxyapatitenanoparticlesosteogenesissilk

More Related Videos

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
09:35

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect

Published on: September 11, 2015

10.2K
Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
09:32

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

Published on: April 19, 2015

10.5K

Related Experiment Videos

Last Updated: Apr 20, 2026

Synthesis of Graphene-Hydroxyapatite Nanocomposites for Potential Use in Bone Tissue Engineering
07:14

Synthesis of Graphene-Hydroxyapatite Nanocomposites for Potential Use in Bone Tissue Engineering

Published on: July 27, 2022

4.3K
Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
09:35

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect

Published on: September 11, 2015

10.2K
Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
09:32

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

Published on: April 19, 2015

10.5K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Silk-based scaffolds are promising for bone regeneration.
  • Incorporating hydroxyapatite (HA) can improve mechanical properties and osteoinductivity.
  • Controlling the nanoscale structure of HA within silk is crucial for optimal performance.

Purpose of the Study:

  • To develop and characterize silk/hydroxyapatite (HA) composite scaffolds using HA/silk core-shell nanoparticles.
  • To evaluate the effect of these nano-engineered scaffolds on rat bone mesenchymal stem cell (rBMSC) proliferation and osteogenic differentiation.
  • To compare the performance of nano-HA/silk scaffolds with conventional HA/silk composites.

Main Methods:

  • Fabrication of silk/HA composite scaffolds via freeze-drying of silk solution containing HA/silk nanoparticles.
  • Characterization of nanoparticle distribution, HA content, and mechanical properties (compressive strength).
  • In vitro culture of rBMSCs on scaffolds, assessing cell proliferation (confocal microscopy, DNA assay) and osteogenic differentiation (gene expression, biochemical assays for calcium and collagen I deposition).

Main Results:

  • HA/silk nanoparticles were uniformly distributed in silk scaffolds at the nanometer scale up to 40% HA content.
  • Scaffolds exhibited significantly improved compressive strength and stiffness with increasing HA/silk nanoparticle content.
  • rBMSCs showed enhanced proliferation and osteogenic differentiation, with increased calcium and collagen I deposition, in nano-HA/silk scaffolds compared to controls.
  • Nano-HA/silk scaffolds demonstrated superior osteogenic properties and mechanical strength over silk/HA composites with HA aggregates.

Conclusions:

  • Silk/HA composite scaffolds fabricated with HA/silk nanoparticles offer improved mechanical properties and enhanced osteogenic capacity for bone regeneration.
  • Nanoscale integration of HA within silk provides a more favorable microenvironment for rBMSC growth and differentiation.
  • This approach represents a promising strategy for developing advanced biomaterials for bone tissue engineering applications.