Ultrastructural analysis of murine hippocampal neural progenitor cells in culture
Related Experiment Video
Updated: Apr 20, 2026
One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice
Published on: February 25, 2014
1Department of Cytology, Bogomoletz Institute of Physiology, Kyiv, Ukraine; Laboratory of Cell and Tissue Cultures, State Institute of Genetic and Regenerative Medicine, Kyiv, Ukraine.
Abstract:
Despite the great number of studies devoted to neural stem/progenitor cell biology, the ultrastructural characteristics of these cells in vitro have not been fully studied. To determine the fine structure of hippocampal neural progenitor cells (NPCs) in culture, mouse fetal hippocampi (E18) were extracted, dissected, and cells were expanded as adherent monolayer culture. Electron microscopy revealed that NPCs had an immature phenotype, with a high nuclear/cytoplasmic ratio, small and scant organelles, underdeveloped endoplasmic reticulum, and many free ribosomes and polysomes. Our results may contribute to a better understanding of the fine structure and physiology of hippocampal NPCs in vitro.
