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B7-H6 expression in non-small cell lung cancers
Xiuqin Zhang1, Guangbo Zhang2, Yan Qin3
1Department of Respiratory Medicine, The First Affiliated Hospital of Soochow University 188 Shizi Street, Suzhou 215006, China ; The First Affiliated Hospital of Jiangnan University 200 Huihe street, Binhu District, Wuxi 214000, China.
Abstract:
B7 family has been known to be a negative regulator of immunity response in patients with lung cancer. B7-H6 as a novel identified member of B7 family is found to trigger natural killer (NK) cell cytotoxicity and cytokine secretion by binding natural cytotoxicity receptor NKp30. Up until now, no investigations have been made about B7-H6 expression in lung cancer. We present the result of the B7-H6 prognostic value in 65 non-small cell lung cancer (NSCLC) tissues and 65 matched adjacent non-tumor tissues by Immunohistochemistry (IHC). Meanwhile, fluorescence activated cell sorter (FACS) analysis was used to detect B7-H6 receptor NKp30 expression in 7 non-small cell lung cancer tissues and 7 adjacent non-tumor tissues. Here, the result showed B7-H6 immunoreactivity in 6/65 (9.23%) lung cancer patients and 4/65 (6.15%) in adjacent non-tumor tissues. No relationship was found between B7-H6 expression and clinic pathological features. Similarly, no relevance was found for NKp30 expression in lung cancer tissues and non-tumor tissues. However, B7-H6 positive carcinomas were significantly correlated with degree of differentiation (P = 0.044). Three year survival rate after operation did not show the prognostic value for B7-H6 expression. Our study suggests that B7-H6 has a limited value as a prognostic marker in the patients of lung cancer.
Insights
B7-H6 expression in lung cancer shows limited prognostic value. While correlated with differentiation, it did not significantly impact survival rates in non-small cell lung cancer patients.
Area of Science:
- Immunology
- Oncology
- Molecular Biology
Background:
- The B7 family negatively regulates immune responses in lung cancer.
- B7-H6, a novel B7 member, interacts with NKp30 to modulate natural killer cell activity.
- Previous research has not investigated B7-H6 expression in lung cancer.
Purpose of the Study:
- To investigate the prognostic value of B7-H6 expression in non-small cell lung cancer (NSCLC).
- To analyze the correlation between B7-H6 expression and clinicopathological features.
- To assess the expression of NKp30, the receptor for B7-H6, in NSCLC tissues.
Main Methods:
- Immunohistochemistry (IHC) was used to detect B7-H6 expression in 65 NSCLC tissues and matched adjacent non-tumor tissues.
- Fluorescence activated cell sorter (FACS) analysis was performed to detect NKp30 expression in 7 NSCLC tissues and matched adjacent non-tumor tissues.
- Statistical analysis was conducted to evaluate correlations with clinicopathological features and survival.
Main Results:
- B7-H6 immunoreactivity was observed in 9.23% of lung cancer tissues and 6.15% of adjacent non-tumor tissues.
- No significant relationship was found between B7-H6 expression and most clinicopathological features or NKp30 expression.
- B7-H6 positive carcinomas showed a significant correlation with the degree of differentiation (P = 0.044).
- Three-year survival rates did not demonstrate prognostic value for B7-H6 expression.
Conclusions:
- B7-H6 expression in NSCLC is relatively low and does not serve as a reliable prognostic marker for overall survival.
- A significant correlation exists between B7-H6 expression and tumor differentiation, suggesting a potential role in tumor progression.
- Further research may explore the specific mechanisms of B7-H6 in lung cancer, beyond its prognostic implications.
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