Related Experiment Video
Updated: Apr 20, 2026
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
Published on: March 21, 2018
In vitro selection of a single-stranded DNA molecular recognition element specific for bromacil
Ryan M Williams1, Amanda R Kulick2, Srilakshmi Yedlapalli3
1Department of Basic Pharmaceutical Sciences, West Virginia University, 1 Medical Center Drive, P.O. Box 9530, Morgantown, WV 26506, USA ; Memorial Sloan Kettering Cancer Center, Molecular Pharmacology & Chemistry Program, 1275 York Avenue, New York, NY 10065, USA.
Abstract:
Bromacil is a widely used herbicide that is known to contaminate environmental systems. Due to the hazards it presents and inefficient detection methods, it is necessary to create a rapid and efficient sensing device. Towards this end, we have utilized a stringent in vitro selection method to identify single-stranded DNA molecular recognition elements (MRE) specific for bromacil. We have identified one MRE with high affinity (K d = 9.6 nM) and specificity for bromacil compared to negative targets of selection and other pesticides. The selected ssDNA MRE will be useful as the sensing element in a field-deployable bromacil detection device.
