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Flow-cytometric analysis of increased IgE uptake by normal eosinophils following activation with PAF-acether and
G M Walsh1, T Nagakura, Y Iikura
1Department of Allergy, National Children's Research Centre, Tokyo, Japan.
Using flow-cytometric analysis and a fluorescein-conjugated anti-IgE antibody, we have investigated whether normal-density human eosinophils will bind greater amounts of native IgE following activation with platelet-activating factor (PAF), leukotriene B4 (LTB4) and histamine. Incubation with PAF resulted in a large dose- and time-dependent increase in IgE binding, optimal at 10(-7) M and 30 min, respectively. LTB4 and histamine gave lower but significant increases in IgE binding. Enhanced binding of IgE by activated normal-density eosinophils is probably a consequence of enhanced Fc epsilon RII receptor expression, suggesting a functional role for this receptor in allergic inflammation.
Using flow-cytometric analysis and a fluorescein-conjugated anti-IgE antibody, we have investigated whether normal-density human eosinophils will bind greater amounts of native IgE following activation with platelet-activating factor (PAF), leukotriene B4 (LTB4) and histamine. Incubation with PAF resulted in a large dose- and time-dependent increase in IgE binding, optimal at 10(-7) M and 30 min, respectively. LTB4 and histamine gave lower but significant increases in IgE binding. Enhanced binding of IgE by activated normal-density eosinophils is probably a consequence of enhanced Fc epsilon RII receptor expression, suggesting a functional role for this receptor in allergic inflammation.