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Updated: Apr 20, 2026

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
Published on: June 15, 2016
Upregulation of TPX2 by STAT3: identification of a novel STAT3 binding site
Rossana Cocchiola1, Caterina Grillo1, Fabio Altieri1
1Department of Biochemical Sciences, Sapienza University of Rome, 00185 Rome, Italy; Istituto Pasteur-Fondazione Cenci Bolognetti, Piazzale Aldo Moro 5, 00185 Roma, Italy.
Abstract:
TPX2, a protein involved in mitosis, is considered a good marker for actively proliferating tissues, highly expressed in a number of cancer cells. We show the presence of high-affinity binding site for STAT3 in the 5'-flanking region of the Tpx2 gene, which is in vivo bound by activated STAT3. A specific STAT3 peptide inhibitor represses the expression of the Tpx2 gene and inhibits the binding of STAT3 to its consensus sequence in human cell lines where STAT3 is activated. These results indicate that activated STAT3 contributes to the over-expression of Tpx2 through the binding to an enhancer site.
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