Related Experiment Video
Updated: Apr 20, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
Cooperative interactions between p53 and NFκB enhance cell plasticity
Alessandra Bisio1, Judit Zámborszky2, Sara Zaccara1
1Laboratory of Transcriptional Networks, Centre for Integrative Biology, CIBIO, University of Trento, Trento, 38123, Italy.
This study reveals a crosstalk between p53 and NFκB transcription factors in breast cancer cells, influencing cell migration and treatment response. This interaction impacts gene expression, potentially altering cancer progression and therapy effectiveness.
Area of Science:
- Molecular Biology
- Cancer Research
- Cell Signaling
Background:
- p53 and NFκB are key transcription factors regulating cell proliferation and survival.
- Their roles in cancer progression are critical and often opposing.
- Investigating their crosstalk in response to chemotherapy within an inflammatory microenvironment is essential.
Purpose of the Study:
- To explore the crosstalk between p53 and NFκB in MCF7 breast cancer cells.
- To understand how chemotherapy and inflammatory stimuli interact to modulate these pathways.
- To identify gene expression changes and their functional consequences.
Main Methods:
- Transcriptome analysis of MCF7 cells treated with doxorubicin (Doxo) and TNF-alpha (TNFα).
- Quantitative PCR (qPCR) validation of gene expression changes.
- Functional assays including cell migration assays.
- Prognostic analysis of gene signatures in breast cancer patient data.
Main Results:
- Combined Doxo+TNFα treatment synergistically up-regulated specific genes involved in cell migration.
- Seven genes (PLK3, LAMP3, ETV7, UNC5B, NTN1, DUSP5, SNAI1) were identified as synergistically up-regulated and dependent on both p53 and NFκB.
- Increased MCF7 cell motility was observed upon Doxo+TNFα treatment.
- A 29-gene signature showed prognostic value in luminal breast cancer.
Conclusions:
- Crosstalk between p53 and NFκB pathways is induced by chemotherapy and inflammatory signals.
- This crosstalk activates gene expression programs that enhance cell migration.
- The identified gene signature has prognostic implications for breast cancer patients.
- Understanding this crosstalk may offer new therapeutic strategies.
More Related Videos
04:56Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence
Published on: December 30, 2025
10:57NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
Published on: January 12, 2020
Related Concept Videos
Abnormal Proliferation
NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The...
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
DNA Damage Can Stall the Cell Cycle
DNA Damage can Stall the Cell Cycle
Negative Regulator Molecules