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Multimodal nonlinear optical microscopy with shaped 10 fs pulses.

Jean Rehbinder, Lukas Brückner, Alexander Wipfler

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    |November 18, 2014
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    We used shaped ultrashort laser pulses for advanced multimodal microscopy. This technique enhances signal intensity and selectivity for clearer biological imaging, improving contrast in samples like skin.

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    Area of Science:

    • * Optics and Photonics
    • * Biophysics
    • * Microscopy

    Background:

    • * Ultrafast lasers are crucial for nonlinear microscopy.
    • * Optimizing laser pulse characteristics can enhance signal generation and selectivity.
    • * Multimodal microscopy combines different nonlinear signals for comprehensive sample analysis.

    Purpose of the Study:

    • * To demonstrate the utility of shaped 10 femtosecond (fs) laser pulses for multimodal microscopy.
    • * To explore how tailored pulse waveforms can optimize nonlinear optical signals for improved imaging.
    • * To showcase the flexibility of a broadband laser source coupled with a pulse shaper.

    Main Methods:

    • * Employed a broadband oscillator and an external pulse shaper to generate tunable 10 fs laser pulses.
    • * Utilized shaped pulses to excite various nonlinear optical phenomena.
    • * Performed multimodal imaging using coherent anti-Stokes Raman scattering (CARS), two-photon fluorescence (2PF), and second harmonic generation (SHG).

    Main Results:

    • * Demonstrated that shaped pulses can be optimized for either signal intensity or selectivity.
    • * Showcased that complex waveforms improve contrast by targeting specific molecular transitions.
    • * Successfully imaged biological samples, including a moss leaf and human skin, with high contrast.

    Conclusions:

    • * Shaped 10 fs pulses offer a versatile light source for multimodal nonlinear microscopy.
    • * Pulse shaping provides a powerful tool to enhance signal generation efficiency and spectral selectivity.
    • * This approach enables advanced contrast mechanisms for detailed biological sample imaging.