rPLR: an imaging system for measuring pupillary light reflex at a distance

Pupillary light reflex (PLR) is a simple noninvasive neurological test that can reveal a great amount of information of the neural system. We report here a novel imaging system for measuring PLR without using any restraints to limit the subject's movement. Our system incorporates a tracking component that can locate the subject's eye position and redirect the pupillary imaging component to follow the subject's movement. This system can measure PLR, at a distance from the subject, with high spatial resolution (<50  μm) and temporal resolution (120 Hz). Because this new PLR device can accommodate the subject's movement, it is well positioned to test in young children and other people who have difficulty remaining voluntarily still during tests.