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De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
Published on: February 18, 2022
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Quantitative profiling of peptides from RNAs classified as noncoding
Sudhakaran Prabakaran1, Martin Hemberg2, Ruchi Chauhan3
11] Proteomics Center, Boston Children's Hospital, Boston, Massachusetts 02115, USA [2] Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Nature Communications
|November 19, 2014
Summary
Researchers discovered novel peptides translated from noncoding RNAs in mouse neurons. These findings reveal a previously unrecognized layer of biological regulation in gene expression and neuronal function.
Area of Science:
- Genomics
- Molecular Biology
- Neuroscience
Background:
- The mammalian genome contains a vast noncoding RNA (ncRNA) fraction, largely assumed to lack protein-coding potential.
- Previous research has not systematically investigated the translation and physiological regulation of ncRNAs.
Purpose of the Study:
- To computationally identify non-canonical translation products from unannotated transcripts in mouse neurons.
- To explore the potential for novel peptide generation from previously non-coding genomic regions.
Main Methods:
- Analysis of unannotated transcripts in mouse neurons using computational methods.
- Integration of transcriptomic data with proteomic data to identify translation products.
- Examination of temporal regulation patterns of novel translation products.
Main Results:
- Identification of non-canonical translation products originating from both intragenic and extragenic genomic regions.
- Evidence for peptides derived from antisense transcripts and introns.
- Demonstration of temporal regulation in novel translation products, mirroring known neuronal activity proteins.
Conclusions:
- This study provides evidence for a significant and complex landscape of biologically regulated translation from transcripts previously considered non-coding.
- These findings suggest a novel layer of gene expression regulation with implications for neuronal function.
- The discovery of peptides from ncRNAs broadens our understanding of the functional potential of the genome.
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