Related Experiment Video

Updated: Apr 20, 2026

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
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Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

Published on: August 2, 2018

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Contribution of phosphoproteomics in understanding SRC signaling in normal and tumor cells

Audrey Sirvent1, Serge Urbach, Serge Roche

  • 1CNRS UMR5237, University Montpellier 1 and 2, CRBM, Montpellier, France.

Proteomics
|November 19, 2014
PubMed

Insights

The SRC tyrosine kinase (SRC) is crucial for cell signaling and growth. Deregulated SRC drives cancer, and new phosphoproteomic insights reveal its mechanisms, aiding targeted cancer therapies.

Area of Science:

  • Cellular Biology
  • Molecular Oncology
  • Biochemistry

Background:

  • The SRC tyrosine kinase (SRC) is a key regulator of signal transduction pathways initiated by cell-surface receptors.
  • SRC plays a critical role in cell growth, migration, and survival.
  • Deregulated SRC activity is implicated in human cancer development and progression.

Purpose of the Study:

  • To review the impact of phosphoproteomic methods on understanding SRC signaling mechanisms.
  • To explore the role of SRC in normal and tumor cells.
  • To discuss therapeutic strategies targeting SRC signaling in cancer.

Main Methods:

  • Review of phosphoproteomic studies identifying SRC substrates.
  • Analysis of SRC's role in receptor tyrosine kinase (RTK) signaling.
  • Integration of findings for therapeutic strategy development.

Main Results:

  • Phosphoproteomics has significantly expanded the known SRC substrate repertoire.
  • SRC promotes RTK-mediated signaling, contributing to oncogenesis even without external stimuli.
  • SRC deregulation is a common event in human cancers.

Conclusions:

  • Novel insights from phosphoproteomics are refining our understanding of SRC signaling pathways.
  • Targeting SRC signaling presents a promising therapeutic avenue for various human cancers.
  • Further research into SRC substrates and regulation is vital for effective cancer treatment.
Keywords:
CancerCell biologyCell signalingPhosphoproteomicsSRCTyrosine kinase

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