Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Utility of microbial cell-free DNA sequencing in the diagnosis of mycobacterial infections in a quaternary care center by Centeno et al.

Antimicrobial stewardship & healthcare epidemiology : ASHE·2026
Same author

Analytical and clinical validation of direct detection of antimicrobial resistance markers by plasma microbial cell-free DNA sequencing.

Journal of clinical microbiology·2024
Same author

Cost-Effectiveness of Plasma Microbial Cell-Free DNA Sequencing When Added to Usual Care Diagnostic Testing for Immunocompromised Host Pneumonia.

PharmacoEconomics·2024
Same author

Detection of Mpox Virus Using Microbial Cell-Free DNA: The Potential of Pathogen-Agnostic Sequencing for Rapid Identification of Emerging Pathogens.

The Journal of infectious diseases·2023
Same author

Plasma Microbial Cell-Free DNA Sequencing from over 15,000 Patients Identified a Broad Spectrum of Pathogens.

Journal of clinical microbiology·2023
Same author

Proceedings of the Clinical Microbiology Open 2018 and 2019 - a Discussion about Emerging Trends, Challenges, and the Future of Clinical Microbiology.

Journal of clinical microbiology·2022

Related Experiment Video

Updated: Apr 20, 2026

Author Spotlight: Optimizing Digital Droplet PCR Method for Accurate Adeno-Associated Viral Genome Quantification
04:43

Author Spotlight: Optimizing Digital Droplet PCR Method for Accurate Adeno-Associated Viral Genome Quantification

Published on: October 11, 2024

2.7K

Quantitative nucleic acid amplification methods for viral infections.

Jonathan C Gullett1, Frederick S Nolte2

  • 1Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC.

Clinical Chemistry
|November 19, 2014
PubMed
Summary
This summary is machine-generated.

Quantitative nucleic acid amplification tests improve viral load measurement for diagnosing and managing viral infections. Digital PCR offers a promising solution to current challenges in accuracy and standardization for viral load testing.

More Related Videos

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
08:36

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Published on: November 1, 2018

32.8K
Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
08:37

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

14.9K

Related Experiment Videos

Last Updated: Apr 20, 2026

Author Spotlight: Optimizing Digital Droplet PCR Method for Accurate Adeno-Associated Viral Genome Quantification
04:43

Author Spotlight: Optimizing Digital Droplet PCR Method for Accurate Adeno-Associated Viral Genome Quantification

Published on: October 11, 2024

2.7K
Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
08:36

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Published on: November 1, 2018

32.8K
Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
08:37

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

14.9K

Area of Science:

  • Virology
  • Molecular Diagnostics
  • Clinical Laboratory Science

Background:

  • Significant advancements in quantifying viral nucleic acid in bodily fluids over the past two decades.
  • Viral load measurements are integral to diagnostic and treatment guidelines for numerous viral infections.
  • These quantitative methods are widely implemented in diverse clinical settings.

Purpose of the Study:

  • To describe quantitative nucleic acid amplification methods for viral load measurement.
  • To highlight key performance variables and clinical applications of these methods.
  • To examine current challenges and future directions in viral load testing.

Main Methods:

  • Review of quantitative nucleic acid amplification techniques.
  • Focus on US Food and Drug Administration-cleared and laboratory-developed tests.
  • Discussion of clinical applications for HIV-1, HCV, HBV, CMV, EBV, and BK polyomavirus.

Main Results:

  • Quantitative nucleic acid amplification tests provide crucial data for predicting disease progression.
  • These tests help differentiate symptomatic from asymptomatic infections.
  • Efficacy of antiviral therapies can be assessed using viral load measurements.

Conclusions:

  • Despite technological progress, challenges in accuracy, precision, and standardization persist in viral load testing.
  • Digital PCR, a direct quantification method, shows potential to overcome current limitations.
  • Advances in viral load quantification enhance patient management and therapeutic monitoring.