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Updated: Apr 20, 2026
Isolation and Cellular Phenotyping of Mesenchymal Stem Cells Derived from Synovial Fluid and Bone Marrow of Minipigs
Published on: July 2, 2016
Mouse synovial mesenchymal stem cells increase in yield with knee inflammation
Yu Matsukura1, Takeshi Muneta, Kunikazu Tsuji
1Department of Joint Surgery and Sports Medicine, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
Abstract:
Even though mouse studies have various advantages, harvesting an adequate number of synovial mesenchymal stem cells (MSCs) is difficult in mice. We investigated whether the total yield of MSCs increased in synovium with inflammation in mice. Infrapatellar fat pads (IFPs) were harvested from 10 knees of 5 mice 3, 7, and 14 days after intraarticular injection of carrageenan. Ten IFPs were also harvested from untreated knees as a control. Seven days after initial plating, the total yield of cells was compared among the 4 groups (n = 4-6). The harvested cells were analyzed for multipotentiality and surface epitopes. Furthermore, knee synovitis was compared among the 4 groups in histology. The number of cells in the 3 and 7 days treated group was significantly higher than the other groups. The harvested cells had characteristics of MSCs. Synovitis in the 3 and 7 days treated groups was significantly severer than the other groups. There seemed to be a relationship between the synovitis score and the total yield of cells derived from IFPs. In mice, it became possible to increase the yield 50-fold by inducing inflammation. This method makes it possible to analyze the molecular mechanisms of cartilage regeneration of synovial MSCs in mice models.
Insights
Inflammation in mouse knee joints significantly boosts the yield of mesenchymal stem cells (MSCs) from infrapatellar fat pads. This 50-fold increase aids in studying cartilage regeneration mechanisms.
Area of Science:
- Biomedical Engineering
- Stem Cell Biology
- Musculoskeletal Research
Background:
- Mesenchymal stem cells (MSCs) are crucial for tissue regeneration.
- Harvesting sufficient MSCs from mouse synovium is challenging for research.
- Inflammation's effect on synovial MSC yield in mice is not well understood.
Purpose of the Study:
- To determine if induced synovitis increases the yield of synovial mesenchymal stem cells (MSCs) from mouse infrapatellar fat pads (IFPs).
- To characterize the harvested cells and assess the relationship between inflammation severity and cell yield.
Main Methods:
- Infrapatellar fat pads (IFPs) were harvested from mouse knees at 3, 7, and 14 days post-carrageenan injection.
- Control group consisted of IFPs from untreated knees.
- Cell yield, multipotency, surface epitopes, and synovitis severity (histology) were analyzed.
Main Results:
- A significant 50-fold increase in cell yield was observed in the 3- and 7-day post-treatment groups compared to controls.
- Harvested cells exhibited characteristics of MSCs.
- Increased cell yield correlated with higher synovitis scores.
Conclusions:
- Induced synovitis in mice effectively increases the yield of synovial MSCs from IFPs.
- This method facilitates the study of molecular mechanisms underlying cartilage regeneration using synovial MSCs in mouse models.
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