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Interaction of the CRP-cAMP complex with the cea regulatory region
1Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston 77225.
Molecular & General Genetics : MGG
|February 1, 1989
Abstract:
Analysis of the induction of expression of cea-lacZ fusions in cya and crp mutants showed that catabolite repression affects the kinetics of induction and the rate of induced synthesis. In a cya mutant, addition of cAMP reduced the induction lag and increased the amount of beta-galactosidase produced. The CRP-cAMP complex was found to bind to two sites 5' to the cea promoter, but deletion analysis showed that only one of these was involved in the control of cea. Deletion of this site resulted in a loss of the stimulatory effects of cAMP in a cya mutant.