Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

Atomic Fluorescence Spectroscopy

Atomic Fluorescence Spectroscopy

Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...

Studying the Cytoskeleton

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Clonal evolution and mutational trajectories of metastatic colorectal cancer shaped by anticancer therapies.

Nature communications·2026

Same author

Evaluating the Effect of Sorafenib on Gd-EOB-DTPA-mediated Contrast Enhancement: An Experimental Study using DCE-MRI.

Molecular imaging and biology·2026

Same author

3-Dimensional reconstruction reveals frequent intraluminal growth of submucosal veins in surgically resected pT1 colorectal cancers.

Journal of pathology and translational medicine·2026

Same author

Tumor Exosomal L1 Cell Adhesion Molecule Promotes Brain Metastasis of Lung Cancer.

Research (Washington, D.C.)·2026

Same author

Long-term conditional relative survival trends in extrahepatic bile duct cancer: insights from a population-based retrospective cohort study in a high-incidence Asian Country, 1999-2021.

International journal of surgery (London, England)·2026

Same author

Yeast-derived β-glucan enhances angiogenesis by activating the AMPK-HDAC7-MEF2 axis.

International journal of biological macromolecules·2026

Search research articles

Related Experiment Video

Updated: Apr 20, 2026

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

Dynamic and unique nucleolar microenvironment revealed by fluorescence correlation spectroscopy.

Hweon Park¹, Sung-Sik Han², Yasushi Sako¹

¹*Department of Life Sciences, Korea University, Seoul, Republic of Korea; Cellular Informatics Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama, Japan; and Asan Institute for Life Sciences, University of Ulsan, College of Medicine, Asan Medical Center, Seoul, Republic of Korea.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology

|November 19, 2014

Summary

Molecular crowding in the nucleolus influences protein mobility, affecting its organization and function. This effect is dependent on cellular energy and transcription processes.

Keywords:

ATP depletion actinomycin D crowding effect diffusion coefficient nucleolar organization

More Related Videos

Author Spotlight: Comprehensive Epigenetic Analysis for Investigating Human Cellular Plasticity and Environmental Adaptation Using Immunofluorescence Assays

Author Spotlight: Comprehensive Epigenetic Analysis for Investigating Human Cellular Plasticity and Environmental Adaptation Using Immunofluorescence Assays

Published on: June 28, 2024

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Published on: September 6, 2024

Related Experiment Videos

Last Updated: Apr 20, 2026

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

Author Spotlight: Comprehensive Epigenetic Analysis for Investigating Human Cellular Plasticity and Environmental Adaptation Using Immunofluorescence Assays

Author Spotlight: Comprehensive Epigenetic Analysis for Investigating Human Cellular Plasticity and Environmental Adaptation Using Immunofluorescence Assays

Published on: June 28, 2024

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Published on: September 6, 2024

Area of Science:

Cell Biology
Biophysics

Background:

Nucleolar organization and function rely on factor mobility and interactions.
Molecular crowding is crucial in dense structures like the nucleolus but not fully understood.

Purpose of the Study:

To investigate the biophysical properties of nucleolar organization.
To understand molecular crowding effects within the nucleolus.

Main Methods:

Analyzed diffusional behaviors of inert green fluorescent protein (GFP) oligomers.
Utilized fluorescence correlation spectroscopy under various conditions.
Investigated GFP mobility with and without nuclear localization signals (NLSs).

Main Results:

GFP mobility in the nucleolus and nucleoplasm follows free diffusion, though slower in the nucleolus.
GFP diffusion in the nucleolus is size- and NLS-dependent.
Nucleolar mobility is sensitive to ATP depletion and actinomycin D (ActD) treatment, unlike nucleolar ultrastructure.

Conclusions:

The nucleolus acts like an aqueous medium with significant molecular crowding.
Nucleolar crowding is influenced by cellular energy (ATP) and transcription.
ActD treatment alters nucleolar structure, suggesting a role for transcription in maintaining organization.