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Switchavidin: reversible biotin-avidin-biotin bridges with high affinity and specificity
Barbara Taskinen1, Dominik Zauner, Soili I Lehtonen
1BioMediTech, University of Tampere , Biokatu 6, FI-33520 Tampere, Finland.
Bioconjugate Chemistry
|November 19, 2014
Abstract:
Switchavidin is a chicken avidin mutant displaying reversible binding to biotin, an improved binding affinity toward conjugated biotin, and low nonspecific binding due to reduced surface charge. These properties make switchavidin an optimal tool in biosensor applications for the reversible immobilization of biotinylated proteins on biotinylated sensor surfaces. Furthermore, switchavidin opens novel possibilities for patterning, purification, and labeling.

