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Characterizing Cell Migration Within Three-dimensional In Vitro Wound Environments
Published on: August 16, 2017
Testing Wound-healing Activity in T15 Fibroblast Cultures: A Morphometric Analysis
S Korkmaz1, H Zeytinoglu2, M Zeytinoglu2
1Department of Pharmacology, Faculty of Pharmacy, Anadolu University, Eskisehir, Turkey.
Abstract:
The purpose was to evaluate the use of mouse T15 fibroblast cell cultures for the investigation of wound-healing activity. In order to investigate their mechanisms of action, the effects of drugs with wound-healing activities were compared by using morphometric analyses by microscopy after cell staining. A number of parameters were used to evaluate the effects of titrated extracts from Centella asiatica and dexpanthenol (drugs that have been used in medical practice for their wound-healing activities) on cultured mouse T15 fibroblasts. These parameters were : the total number of cells ; the number of T15 cells in mitosis ; the percentages of fusiform, polygonal, round and vacuole-containing cells ; and the number of intracellular collagen granules. The results indicate that these two drugs exhibit wound-healing activities by activating fibroblast cells, and have cytoprotective effects, although their mechanisms of action on mouse T15 fibroblasts were different. On the basis of our findings, mouse T15 fibroblast cell cultures seem to be useful for the pharmacological screening of compounds with wound-healing activity.
Insights
Mouse T15 fibroblast cell cultures effectively model wound healing. Centella asiatica and dexpanthenol promote fibroblast activation and cytoprotective effects, aiding in pharmacological screening for wound-healing compounds.
Area of Science:
- Pharmacology
- Cell Biology
- Dermatology
Background:
- Fibroblast cells are crucial for wound healing.
- Identifying effective wound-healing agents requires reliable screening models.
- Centella asiatica and dexpanthenol are established treatments with wound-healing properties.
Purpose of the Study:
- To evaluate mouse T15 fibroblast cell cultures as a model for investigating wound-healing activity.
- To compare the mechanisms of action of Centella asiatica and dexpanthenol on fibroblasts.
- To assess the utility of this cell culture model for pharmacological screening.
Main Methods:
- Utilized morphometric analyses via microscopy on stained mouse T15 fibroblasts.
- Assessed parameters including cell count, mitosis, cell morphology (fusiform, polygonal, round, vacuole-containing), and intracellular collagen granules.
- Compared the effects of titrated extracts from Centella asiatica and dexpanthenol.
Main Results:
- Both Centella asiatica and dexpanthenol demonstrated wound-healing activities by activating fibroblast cells.
- The tested agents exhibited cytoprotective effects on the cultured fibroblasts.
- Distinct mechanisms of action were observed between Centella asiatica and dexpanthenol on T15 fibroblasts.
Conclusions:
- Mouse T15 fibroblast cell cultures serve as a valuable tool for the pharmacological screening of compounds with wound-healing potential.
- The study validates the use of this model for understanding the mechanisms of wound-healing agents.
- Fibroblast activation and cytoprotection are key indicators of wound-healing efficacy in this model.
