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Intravenous Injections in Neonatal Mice
Published on: November 11, 2014
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Intravenous injections in neonatal mice
Sara E Gombash Lampe1, Brian K Kaspar2, Kevin D Foust3
1Department of Neuroscience, Ohio State University.
Journal of Visualized Experiments : Jove
|November 20, 2014
Summary
A novel intravenous injection technique targets the temporal vein in neonatal mice for efficient therapeutic delivery. This method, viable for the first two days after birth, enables stable, body-wide transgene expression using adeno-associated virus vectors.
Area of Science:
- * Neonatal mouse models
- * Gene therapy delivery
Background:
- * Intravenous injections are standard for adult animals but challenging in rapidly progressing neonatal mouse disease models.
- * Early-onset diseases in mice necessitate effective therapeutic delivery methods in early life stages.
Purpose of the Study:
- * To establish a reliable intravenous injection method for neonatal mice.
- * To enable early and efficient delivery of therapeutics, such as adeno-associated virus (AAV) vectors.
Main Methods:
- * Utilized the temporal vein, visible anterior to the ear bud in neonatal mice within the first two days post-birth.
- * Administered up to 50 μl of therapeutic solution via dissecting microscope guidance.
- * Procedure completed within 1-2 minutes, with pups safely returned to dams.
Main Results:
- * The temporal vein injection technique is safe and well-tolerated in neonatal mice.
- * This method allows for effective delivery of adeno-associated virus (AAV) vectors.
- * Achieved near body-wide and stable transgene expression, dependent on AAV serotype.
Conclusions:
- * Neonatal temporal vein injection offers a safe and effective route for therapeutic delivery in mice.
- * This technique overcomes challenges in treating early-onset, rapidly progressing mouse models.
- * Enables long-term therapeutic effects through stable transgene expression via AAV vectors.
