Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

19.7K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
19.7K
Cascaded Op Amps01:16

Cascaded Op Amps

1.3K
Operational amplifiers (op-amps) are versatile electronic components that can be interconnected in a cascade - one after another in a linear sequence. This cascading is possible due to their infinite input resistance and zero output resistance, allowing them to maintain their input-output relationships even when connected in series.
In a cascaded system, each op-amp is referred to as a stage. The output of one stage drives the input of the subsequent stage. As the input signal passes through...
1.3K
PCR01:32

PCR

243.2K
Overview
243.2K
Instrumentation Amplifier01:25

Instrumentation Amplifier

1.3K
An electrocardiography (ECG) machine is an essential piece of medical equipment used to monitor the electrical activity of the heart. It operates by detecting small electrical changes on the skin that result from the depolarization of the heart muscle during each heartbeat. However, these signals are in the microvolt range and can be easily overwhelmed by noise or interference.
To overcome this challenge, an ECG machine utilizes an instrumentation amplifier. This specialized amplifier is...
1.3K
Design Example: Vintage Mixing Console01:17

Design Example: Vintage Mixing Console

665
A sound engineer at a music company recently encountered a problem. The output from their newly acquired studio's vintage mixing console was too low for the requirements of modern recording equipment. To rectify this situation, the engineer decided to design an audio pre-amplifier using an operational amplifier (op-amp) to boost the signal level.
The specifications for the pre-amplifier were clear. It needed to amplify the audio signal by a factor of 10, have an input impedance above 10...
665
PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

113.2K
113.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of small-molecule HSF1 amplifiers by high content screening in protection of cells from stress induced injury.

Biochemical and biophysical research communications·2009
Same author

Nanowire transformation by size-dependent cation exchange reactions.

Nano letters·2009
Same author

Effect of haishengsu as an adjunct therapy for patients with advanced renal cell cancer: a randomized and placebo-controlled clinical trial.

Journal of alternative and complementary medicine (New York, N.Y.)·2009
Same author

Identification of inhibitors of HSF1 functional activity by high-content target-based screening.

Journal of biomolecular screening·2009
Same author

Antitumor effects of targeting hTERT lentivirus-mediated RNA interference against KB cell lines.

Oncology research·2009
Same author

Characteristics of emissive spectrum and the removal of nitric oxide in N2/02/NO plasma with argon additive.

Journal of environmental sciences (China)·2009
Same journal

A two-step centrifugal microfluidic platform for semi-automated IGRA detection of tuberculosis based on chemiluminescence.

The Analyst·2026
Same journal

On-site rapid identification of animal and plant creams <i>via</i> 2D FeB nanozyme-based colorimetric sensors.

The Analyst·2026
Same journal

Sensitive detection of aflatoxin B1 using a dual-mode fluorescent aptasensor based on cascade signal amplification.

The Analyst·2026
Same journal

Deep learning-enabled microfluidic digital PCR platform for efficient seven-color quantification.

The Analyst·2026
Same journal

Monitoring food spoilage biogenic amines utilizing a blue-emitting fluorescent ionic liquid.

The Analyst·2026
Same journal

Correction: Regeneration-on-a-chip: a planarian microfluidic device enabling automated cultivation, individual tracking and <i>in vivo</i> imaging for regeneration study.

The Analyst·2026
See all related articles

Related Experiment Video

Updated: Apr 20, 2026

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems
07:35

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

Published on: June 14, 2021

3.3K

A netlike rolling circle nucleic acid amplification technique.

Xiaoli Zhu1, Chang Feng, Bin Zhang

  • 1Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, P R China.

The Analyst
|November 20, 2014
PubMed
Summary
This summary is machine-generated.

A novel nucleic acid amplification method, netlike rolling circle amplification, was developed. This technique enables highly sensitive detection of Human Immunodeficiency Virus (HIV) sequences through cubic amplification.

More Related Videos

Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples
10:13

Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples

Published on: December 2, 2022

3.2K
Increasing cDNA Yields from Single-cell Quantities of mRNA in Standard Laboratory Reverse Transcriptase Reactions using Acoustic Microstreaming
04:52

Increasing cDNA Yields from Single-cell Quantities of mRNA in Standard Laboratory Reverse Transcriptase Reactions using Acoustic Microstreaming

Published on: July 11, 2011

10.7K

Related Experiment Videos

Last Updated: Apr 20, 2026

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems
07:35

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

Published on: June 14, 2021

3.3K
Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples
10:13

Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples

Published on: December 2, 2022

3.2K
Increasing cDNA Yields from Single-cell Quantities of mRNA in Standard Laboratory Reverse Transcriptase Reactions using Acoustic Microstreaming
04:52

Increasing cDNA Yields from Single-cell Quantities of mRNA in Standard Laboratory Reverse Transcriptase Reactions using Acoustic Microstreaming

Published on: July 11, 2011

10.7K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Nucleic Acid Amplification

Background:

  • Rolling circle amplification (RCA) is a method for amplifying nucleic acids.
  • Hyperbranched RCA (HRCA) offers increased amplification efficiency.
  • Sensitive detection of specific nucleic acid sequences is crucial for diagnostics.

Purpose of the Study:

  • To develop a novel nucleic acid amplification technique.
  • To enhance the sensitivity and efficiency of existing RCA methods.
  • To enable sensitive detection of Human Immunodeficiency Virus (HIV) sequences.

Main Methods:

  • Introduction of a nicking enzyme into the hyperbranched rolling circle amplification (HRCA) system.
  • Development of netlike rolling circle amplification (NRCA).
  • Utilizing NRCA for sensitive sequence detection.

Main Results:

  • Achieved a dense and uniform network morphology.
  • Demonstrated cubic amplification.
  • Successfully detected a specific sequence from HIV with high sensitivity.

Conclusions:

  • Netlike rolling circle amplification (NRCA) is an effective nucleic acid amplification technique.
  • NRCA offers significant advantages in sensitivity and efficiency over existing methods.
  • The developed method shows promise for sensitive HIV detection.